Isolation and Culture of Panning Method‐enriched Langerhans Cells from Dispase‐dissociated Epidermal Cells of the Mouse
Isolation and Culture of Panning Method‐enriched Langerhans Cells from Dispase‐dissociated Epidermal Cells of the Mouse
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从分散酶解离的小鼠表皮细胞中分离和培养淘选方法富集的朗格汉斯细胞
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
T. Marunouchi
中科院分区：
文献类型：
--
作者：
Y. Koyama;Miya Kobayashi;K. Ohashi;S. Nagao;J. Niwa;Hisahide Takahashi;T. Hoshino;T. Marunouchi
朗格汉斯细胞（LC）是骨髓来源的，表皮中的Ia阳性抗原呈递细胞，占总表皮细胞的2-4%。我们检查了分散酶处理和淘选方法的组合用于富集和培养小鼠LC的有用性。用部分纯化的分散酶（Godo Shusei，II型）处理躯干皮肤以分离表皮片并解离表皮细胞。用含有抗Ia单克隆抗体的腹水或培养上清液处理悬浮细胞，并通过Ia介导的淘选方法富集LC。每只小鼠回收3-4 × 105个LC，纯度>95%，活力>90%。富集的LC有效刺激同种异体混合白细胞反应。超微结构观察显示，丰富的LC含有许多囊泡，但几乎没有Birbeck颗粒。当腹水用作抗Ia抗体时，观察到明显粘附于LC表面的层状结构。这些结果表明，分散酶处理和Ia介导的淘选方法的组合对于分离高产量的具有高纯度和活力的功能成熟鼠朗格汉斯细胞非常有用。
Langerhans cells (LCs) are bone marrow‐derived, Ia‐positive antigen‐presenting cells in the epidermis which constitute 2–4% of the total epidermal cells. We examined the usefulness of a combination of dispase treatment and the panning method for enriching and culturing mouse LCs. Trunk skin was treated with partially purified dispase (Godo Shusei, type II) to separate epidermal sheets and to dissociate epidermal cells. Suspended cells were treated with ascites or culture supernatant containing anti‐Ia monoclonal antibody, and LCs were enriched by the Ia‐mediated panning method. Per mouse, 3–4 × 105 LCs were recovered with >95% purity and >90% viability. Enriched LCs potently stimulated the allogeneic mixed‐leukocyte reaction. Ultrastructural observations revealed that enriched LCs contained many vesicles but almost no Birbeck granules. A laminal structure, which was apparently adhesive to the surface of LCs, was observed when ascites were employed as the anti‐Ia antibody. These results indicate that a combination of dispase treatment and the Ia‐mediated panning method is very useful for isolating high yields of functionally mature murine Langerhans cells with high purity and viability.
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影响因子：
6.5
作者：
STENN, KS;LINK, R;KUKLINSKA, E
通讯作者：
KUKLINSKA, E
培养的鼠表皮朗格汉斯细胞表面抗原的定量：表面 MHC 产物水平的快速和选择性增加。
DOI：
10.1111/1523-1747.ep12456460
发表时间：
1988
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Witmer-Pack,MD;Valinsky,J;Olivier,W;Steinman,RM
通讯作者：
Steinman,RM
使用泛白细胞单克隆抗体通过固相免疫吸收富集小鼠和人类朗格汉斯细胞。
DOI：
10.1111/1523-1747.ep12274673
发表时间：
1985
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Wood,GS;Kosek,J;Butcher,EC;Morhenn,VB
通讯作者：
Morhenn,VB
小鼠表皮树突状细胞的纯化和鉴定：流式细胞术和免疫金标记。
DOI：
10.1177/35.7.2884251
发表时间：
1987
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Picut,CA;Lee,CS;Dougherty,EP;Andersen,KL;Lewis,RM
通讯作者：
Lewis,RM
高度富集的朗格汉斯细胞的体外免疫刺激能力。
DOI：
10.1111/1523-1747.ep12462221
发表时间：
1988
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Picut,CA;Lee,CS;Dougherty,EP;Anderson,KL;Lewis,RM
通讯作者：
Lewis,RM