Interleukin-27 gene delivery for modifying malignant interactions between prostate tumor and bone.
Interleukin-27 gene delivery for modifying malignant interactions between prostate tumor and bone.
复制标题
Interleukin-27 基因传递可改变前列腺肿瘤与骨之间的恶性相互作用。
作者：
Olga Zolochevska;Jayne Ellis;S. Parelkar;Delphine Chan;T. Emrick;Jingna Wei;I. Patrikeev;M. Motamedi;M. Figueiredo
我们研究了一种新的细胞因子白细胞介素-27（IL-27）在介导前列腺癌和骨之间相互作用中的作用。IL-27是异源二聚体IL-12相关细胞因子家族中最近表征的成员，并且在几种癌症模型（包括前列腺癌）中显示出阻止肿瘤生长和介导肿瘤消退的前景。前列腺癌通常与骨转移有关，其中肿瘤诱导与成骨细胞和破骨细胞的恶性循环，以诱导骨病变，这是患者疼痛和泌尿系统相关事件的重要原因，包括高骨折风险。我们描述了IL-27基因对前列腺癌细胞、成骨细胞和破骨细胞在不同分化阶段的影响。我们应用了IL-27基因递送方案，在体内利用声致穿孔（sonodeferration），目的是治疗和减少前列腺癌在体内骨转移部位的生长。我们使用了一种新的免疫活性前列腺腺癌模型，并表征了肿瘤生长减少、基因表达和效应细胞谱。我们的研究结果表明，IL-27可以有效地减少肿瘤生长，可以帮助正常化骨结构，并可以促进前列腺肿瘤中效应细胞的积累。这些结果是有希望的，因为它们与开发一种新的基于IL-27的策略相关，该策略可以通过使用这种简单有效的超声递送方法治疗前列腺肿瘤骨转移来治疗肿瘤和骨。
We have examined the role of a novel cytokine, interleukin-27 (IL-27), in mediating interactions between prostate cancer and bone. IL-27 is the most recently characterized member of the family of heterodimeric IL-12-related cytokines and has shown promise in halting tumor growth and mediating tumor regression in several cancer models, including prostate cancer. Prostate cancer is frequently associated with metastases to the bone, where the tumor induces a vicious cycle of communication with osteoblasts and osteoclasts to induce bone lesions, which are a significant cause of pain and skeletal-related events for patients, including a high fracture risk. We describe our findings in the effects of IL-27 gene delivery on prostate cancer cells, osteoblasts, and osteoclasts at different stages of differentiation. We applied the IL-27 gene delivery protocol in vivo utilizing sonoporation (sonodelivery) with the goal of treating and reducing the growth of prostate cancer at a bone metastatic site in vivo. We used a new model of immune-competent prostate adenocarcinoma and characterized the tumor growth reduction, gene expression, and effector cellular profiles. Our results suggest that IL-27 can be effective in reducing tumor growth, can help normalize bone structure, and can promote enhanced accumulation of effector cells in prostate tumors. These results are promising, because they are relevant to developing a novel IL-27-based strategy that can treat both the tumor and the bone, by using this simple and effective sonodelivery method for treating prostate tumor bone metastases.
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影响因子：
--
作者：
Kalliolias, George D.;Zhao, Baohong;Triantafyllopoulou, Antigoni;Park-Min, Kyung-Hyun;Ivashkiv, Lionel B.
通讯作者：
Ivashkiv, Lionel B.
前列腺癌骨转移的组织学、免疫表型和组织形态学特征。
影响因子：
--
作者：
Roudier,MartineP;Corey,Eva;True,LawrenceD;Hiagno,CelestiaS;Ott,SusanM;Vessell,RobertL
通讯作者：
Vessell,RobertL
影响因子：
4.8
作者：
Zolochevska, Olga;Figueiredo, Marxa L.
通讯作者：
Figueiredo, Marxa L.
DOI：
10.1016/j.juro.2008.04.140
发表时间：
2008-09
期刊：
The Journal of urology
影响因子：
--
作者：
Roudier MP;Morrissey C;True LD;Higano CS;Vessella RL;Ott SM
通讯作者：
Ott SM
影响因子：
4.1
作者：
Johnson, Lindsay C.;Johnson, Rachelle W.;Munoz, Steve A.;Mundy, Gregory R.;Peterson, Todd E.;Sterling, Julie A.
通讯作者：
Sterling, Julie A.