The influence of varying proportions of terrestrial and marine dietary protein on the stable carbon-isotope compositions of pig tissues from a controlled feeding experiment

The influence of varying proportions of terrestrial and marine dietary protein on the stable carbon-isotope compositions of pig tissues from a controlled feeding experiment
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控制饲养实验中不同比例的陆地和海洋膳食蛋白质对猪组织稳定碳同位素组成的影响

DOI:
10.1080/20548923.2016.1275477
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发表时间:
2017
影响因子:
3.5
通讯作者:
Webb E
Webb E
中科院分区:
--
文献类型:
--
作者:
Webb E

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近年来,很明显,我们的能力存在局限性,有意义地评估古生物利用稳定同位素组成。这些局限性的出现部分是因为许多关于组织饮食关系的基本假设知之甚少。为了弥补这一不足,进行了一项对照饲养实验,以确定陆地与海洋来源的膳食蛋白质消耗对消费者组织碳同位素组成(δ 13 C)的影响。两代猪饲养的五种饲料与不同比例的陆地(大豆)和海洋(鱼粉)蛋白。对49头猪的组织和体液进行了δ 13 C分析,发现组织-全饲料和组织-饲料蛋白质碳同位素偏移量高度依赖于饲料中海洋蛋白质的百分比。我们认为,δ 13 C抵消的趋势最有可能来自非必需氨基酸的增加,特别是甘氨酸,随着饮食中海洋蛋白质比例的增加。这些发现表明,单独使用散装δ 13 C组成不仅掩盖了相当多的饮食信息,而且还可能导致过去海洋和陆地资源消耗的错误表示。
In recent years, it has become evident that limitations exist in our ability to meaningfully assess palaeodiet using stable isotope compositions. These limitations in part arise because many of the fundamental assumptions about tissue-diet relationships are poorly understood. In order to redress this deficiency, a controlled feeding experiment was undertaken to define the impact of terrestrial- vs. marine-derived dietary protein consumption on consumer tissue carbon isotopic compositions (δ13C). Two generations of pigs were raised on one of five feeds with varying proportions of terrestrial (soy) and marine (fish meal) protein. A comprehensive range of tissues and fluids from 49 pigs was submitted forδ13C analysis.The observed tissue–whole diet and tissue–dietary protein carbon isotopic offsets were found to be highly dependent on the percentage of marine protein in diet. We suggest that the trend inδ13C offsets most likely derives from the increased routing of non-essential amino acids, especially glycine, with the increasing proportion of marine protein in the diet. These findings demonstrate that solely using bulkδ13C compositions not only masks considerable information about diet, but may also lead to erroneous representations of marine and terrestrial resource consumption in the past.
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