Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
复制标题
DOI：
10.1016/j.rbmo.2012.07.001
复制
发表时间：
2012-10
影响因子：
4
通讯作者：
Sung LY
中科院分区：
文献类型：
--
作者：
Chen CH;Xu J;Chang WF;Liu CC;Su HY;Chen YE;Du F;Sung LY
This study documents the spatial and temporal distribution of Oct-4, Cdx-2 and acetylated H4K5 (H4K5ac) by immunocytochemistry staining using in-vivo-derived rabbit embryos at different stages: day-3 compact morulae, day-4 early blastocysts, day-4 expanded blastocysts, day-5 blastocysts, day-6 blastocysts and day-7 blastocysts. The Oct-4 signal was stronger in the inner cell mass (ICM)/epiblast cells than in the trophectoderm (TE) cells in all blastocyst stages except day-4 expanded blastocysts, where the signal was similarly weak in both the ICM and TE cells. The Cdx-2 signal was first detected in a small number of TE cells of day-4 early blastocysts, and became evident in the TE cells exclusively afterwards. A consistently strong H4K5ac signal was observed in the TE cells in all blastocyst stages examined. In particular, this signal was stronger in the TE than in the ICM cells in day-4 early blastocysts, day-4 expanded blastocysts and day-5 blastocysts. Double staining of H4K5ac with either Oct-4 or Cdx-2 on embryos at different blastocyst stages confirmed these findings. This work suggests that day 4 is a critical timing for lineage formation in rabbit embryos. A combination of Oct-4, Cdx-2 and H4K5ac can be used as biomarkers to identify different lineage cells in rabbit blastocysts.
登录
查看更多内容
影响因子：
4
作者：
Chen CH;Chang WF;Liu CC;Su HY;Shyue SK;Cheng WT;Chen YE;Wu SC;Du F;Sung LY;Xu J
通讯作者：
Xu J
影响因子：
4.8
作者：
Honda, Arata;Hirose, Michiko;Ogura, Atsuo
通讯作者：
Ogura, Atsuo
影响因子：
11.8
作者：
Chazaud, Claire;Yamanaka, Yojiro;Rossant, Janet
通讯作者：
Rossant, Janet
影响因子：
64.8
作者：
Meissner, A;Jaenisch, R
通讯作者：
Jaenisch, R
影响因子：
10.5
作者：
Jedrusik, Agnieszka;Parfitt, David-Emlyn;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena