Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells.
Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells.
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作者：
Wang X;Gawri R;Lei C;Lee J;Sowa G;Kandel R;Vo N
无机聚磷酸盐（polyP）普遍存在于所有生命形式中，是由重复的正磷酸盐单元组成的线性聚合物。存在于椎间盘组织中，polyP先前被证明可以增加髓核细胞（NP）细胞外基质的产生。然而，息肉对人纤维环（hAF）细胞代谢的影响尚不清楚。在这里，在链长为22 （polyP‐22）的0.5至1 mM polyP存在下培养的hAF细胞显示糖胺聚糖含量、蛋白聚糖和胶原合成以及聚集蛋白和胶原型基因表达增加。1 mM息肉作用hAF细胞后，基质金属蛋白酶1基因表达水平降低，基质金属蛋白酶3基因表达水平升高。暴露于1 mM polyP‐22后72小时，hAF细胞的三磷酸腺苷（ATP）合成也显著增加。因此，息肉在AF细胞中具有合成代谢和生物能量作用，类似于在NP细胞中观察到的效果。综上所述，这些结果表明息肉是一种潜在的能量来源和椎间盘细胞的代谢调节剂。本研究旨在探讨无机多磷酸盐（polyPs）对人纤维环（AF）细胞生物学的影响。这是首次研究息肉对人房颤细胞的影响。polyPs富含多酸酐键，是已知的ATP合成磷酸分子的供体，在人AF细胞中显示基质生成和ATP生成增加，并全面促进合成代谢。
Ubiquitously found in all life forms, inorganic polyphosphates (polyP) are linear polymers of repeated orthophosphate units. Present in intervertebral disc tissue, polyP was previously shown to increase extracellular matrix production in nucleus pulposus (NP) cells. However, the effects of polyP on human annulus fibrosus (hAF) cell metabolism is not known. Here, hAF cells cultured in the presence of 0.5 to 1 mM polyP, chain length 22 (polyP‐22), showed an increase in glycosaminoglycan content, proteoglycan and collagen synthesis, and aggrecan and collagen type 1 gene expression. Gene expression level of matrix metalloproteinases 1 was reduced while matrix metalloproteinases 3 level was increased in hAF cells treated with 1 mM polyP. Adenosine triphosphate (ATP) synthesis was also significantly increased in hAF cell culture 72 hours after the exposure to 1 mM polyP‐22. PolyP thus has both anabolic and bioenergetic effects in AF cells, similar to that observed in NP cells. Together, these results suggest polyP as a potential energy source and a metabolic regulator of disc cells. The current study investigates the effect of inorganic polyphosphates (polyPs) on human annulus fibrosus (AF) cell biology. This is a first study of its kind to investigate the effect of polyPs on human AF cells. polyPs, rich in polyanhydride bonds and known donors of phosphate molecule for ATP synthesis, showed increase in matrix production and ATP generation in human AF cells and overall promoted anabolism.
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影响因子：
5.3
作者：
Ngo K;Patil P;McGowan SJ;Niedernhofer LJ;Robbins PD;Kang J;Sowa G;Vo N
通讯作者：
Vo N
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
4.8
作者：
KUMBLE, KD;KORNBERG, A
通讯作者：
KORNBERG, A
影响因子：
5.3
作者：
Nasto, Luigi A.;Wang, Dong;Robinson, Andria R.;Clauson, Cheryl L.;Ngo, Kevin;Dong, Qing;Roughley, Peter;Epperly, Michael;Huq, Saiful M.;Pola, Enrico;Sowa, Gwendolyn;Robbins, Paul D.;Kang, James;Niedernhofer, Laura J.;Vo, Nam V.
通讯作者：
Vo, Nam V.
影响因子：
2.8
作者：
Lee, Whitaik David;Gawri, Rahul;Kandel, Rita A.
通讯作者：
Kandel, Rita A.