Combined optical coherence phase microscopy and impedance sensing measurements of differentiating adipose derived stem cells
Combined optical coherence phase microscopy and impedance sensing measurements of differentiating adipose derived stem cells
复制标题
分化脂肪干细胞的光学相干相位显微镜和阻抗传感测量相结合
DOI：
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
P. Bagnaninchi
中科院分区：
文献类型：
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作者：
P. Bagnaninchi
在不使用标记剂的情况下监测二维培养中的分化干细胞的兴趣越来越大。在这项研究中，我们探索了一种结合阻抗传感（IS）和光学相干相显微镜（OCPM）的多模态方法的可行性，以监测与脂肪来源干细胞分化为不同谱系相关的主要生物学事件。采用金电极阵列在Mesenpro RS培养基中培养脂肪干细胞。该系统（ECIS，应用生物物理学）连接到由计算机控制的锁相放大器，复杂阻抗由同相和非相电压导出。从500Hz到100khz的多频率测量每2分钟记录一次。光学相干相位显微镜围绕Thorlabs引擎（930nm FWHM: 90nm）构建，并连接到定制的显微镜探针。IS和OCPM成功集成。在阻抗测量期间，电极区域（250um）以1.5um的横向分辨率成像。阻抗传感给出了分化的平均测量，作为电极区域上阻抗的变化，而OCPM提供了电极顶部发生的细胞事件的附加信息。从OCPM中获取的信息将为细胞分化和阻抗变化相关的数学模型提供信息。在这项研究中，我们已经证明了整合两种非侵入性监测技术的可行性，这将有助于设计基于干细胞的筛选分析。
There is a growing interest in monitoring differentiating stem cells in 2D culture without the use of labelling agents. In this study we explore the feasibility of a multimodality method that combines impedance sensing (IS) and optical coherence phase microscopy (OCPM) to monitor the main biological events associated with adipose derived stem cells differentiation into different lineages. Adipose derived stem cells were cultured in Mesenpro RS medium on gold electrode arrays. The system (ECIS, Applied biophysics) is connected to a lock-in amplifier controlled by a computer, and the complex impedance is derived from the in phase and out of phase voltages. Multi-frequency measurements spanning from 500Hz to 100 kHz are recorded every 2 minutes. The Optical coherence phase microscope is build around a Thorlabs engine (930nm FWHM: 90nm) and connected to a custom build microscope probe. The IS and OCPM were successfully integrated. The electrode area (250um) was imaged with a lateral resolution of 1.5um during impedance measurements. Impedance sensing gave an average measurement of differentiation, as a change in impedance over the electrode area, whereas OCPM provides additional information on the cellular events occurring on top of the electrode. The information retrieved from OCPM will feed a mathematical model correlating cellular differentiation and impedance variation. In this study we have demonstrated the feasibility of integrating two non-invasive monitoring techniques that will be instrumental in designing stem cell based screening assays.
影响因子：
3.6
作者：
Joo, C;Akkin, T;de Boer, JE
通讯作者：
de Boer, JE
影响因子：
5.5
作者：
Popescu, Gabriel;Park, YoungKeun;Badizadegan, Kamran
通讯作者：
Badizadegan, Kamran