Survival characteristics and transcriptome profiling reveal the adaptive response of the Brucella melitensis 16M biofilm to osmotic stress.
Survival characteristics and transcriptome profiling reveal the adaptive response of the Brucella melitensis 16M biofilm to osmotic stress.
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生存特征和转录组分析揭示了布鲁氏菌 16M 生物膜对渗透压的适应性反应
DOI：
10.3389/fmicb.2022.968592
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
布鲁氏菌可以生活在恶劣的环境中，包括渗透压。布鲁氏菌如何集体响应渗透压在很大程度上尚未被探索，特别是在生物膜等空间结构的群落中。为了深入了解这种生长模式，我们着手表征布鲁氏菌 16M 生物膜，描述其表型，并在渗透胁迫和控制条件下对生物膜进行比较转录组分析。我们确定，用 1.5 M 氯化钠攻击的细菌聚集、形成团块以及形成生物膜的能力降低；然而，盐胁迫促进了外膜囊泡从生物膜中的释放。结合对渗透胁迫的基因型反应，我们在渗透条件下生长的 B. melitensis 16M 中鉴定出 279 个差异表达基因，与对照条件相比。 69 个基因上调，210 个基因下调。在渗透胁迫下，生物膜的主要变化基因预计涉及鞭毛组装、细胞包膜、翻译、小RNA调节、转运和结合蛋白以及能量代谢。此外，ABC转运蛋白在基因本体论（GO）和京都基因和基因组百科全书（KEGG）途径中得到丰富。我们重点介绍了 12 个与生物膜阶段细菌对渗透胁迫反应相关的重要 ABC 转运蛋白基因，其中包括一个特定位点 BME_RS12880，它介导生物膜中甜菜碱的积累，以消除渗透胁迫。目前的研究结果可以帮助研究人员深入了解羊种布鲁氏菌16M生物膜对渗透压的适应，并为制定控制布鲁氏菌的干预策略提供信息。
Brucella can inhabit hostile environments, including osmotic stress. How Brucella responds collectively to osmotic stress is largely unexplored, particularly in spatially structured communities such as a biofilm. To gain insight into this growth mode, we set out to characterize the Brucella melitensis 16M biofilm, describe its phenotype, and carry out a comparative transcriptomic analysis between biofilms under osmotic stress and control conditions. We determined that the bacteria challenged with 1.5 M NaCl had a reduced ability to aggregate and form clumps and develop a biofilm; however, the salt stress promoted the release of the outer membrane vesicles from the biofilm. Together with the genotypical response to osmotic stress, we identified 279 differentially expressed genes in B. melitensis 16M grown under osmotic conditions compared with control conditions; 69 genes were upregulated and 210 downregulated. Under osmotic stress, the main changed genes of biofilm were predicted to be involved in flagellar assembly, cell envelope, translation, small RNA regulation, transport and binding proteins, and energy metabolism. In addition, the ABC transporter was enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We highlight 12 essential ABC transporter genes associated with a bacterial response to osmotic stress at the biofilm stage, including one specific locus, BME_RS12880, mediating betaine accumulation in biofilms to eliminate osmotic stress. The current study results can help researchers gain insights into B. melitensis 16M biofilm adaptation to osmotic stress and provide information for developing intervention strategies to control Brucella.
影响因子：
1.8
作者：
Ferooz J;Letesson JJ
通讯作者：
Letesson JJ