Adaptive responses of marine diatoms to zinc scarcity and ecological implications.
Adaptive responses of marine diatoms to zinc scarcity and ecological implications.
复制标题
DOI：
10.1038/s41467-022-29603-y
复制
发表时间：
2022-04-14
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
溶解的海洋表面浓度的基本藻类微量营养素锌表明，锌可能会影响浮游植物，如硅藻，这是海洋初级生产力的主要贡献者的增长。然而，硅藻适应锌缺乏的具体机制知之甚少。利用全局蛋白质组学分析，我们确定了两个蛋白质（ZCRP-A/B，锌/钴反应蛋白A/B）中的四种硅藻物种，成为丰富的锌/钴限制。使用反向遗传技术和同源性数据的表征表明，ZCRP-A（COG 0523结构域蛋白）和ZCRP-B，分别假定锌/钴分子伴侣和膜结合转运复合物组分的作用。沿着太平洋样带的ZCRPs的元蛋白质组学检测显示，在dZn和dCo最稀缺的表面（<200 m）处，ZCRPs的丰度增加，这意味着海藻中的Zn营养胁迫比以前认识到的更为普遍。这些结果表明，部署在太平洋低锌地区的海洋硅藻锌稀缺的多重适应性反应。在本文中，作者鉴定了海洋硅藻中的两种锌/钴响应蛋白（ZCRPs），确定了它们在微量金属运输和体内平衡中的功能作用，以及它们作为海洋锌胁迫指标的应用。
Scarce dissolved surface ocean concentrations of the essential algal micronutrient zinc suggest that Zn may influence the growth of phytoplankton such as diatoms, which are major contributors to marine primary productivity. However, the specific mechanisms by which diatoms acclimate to Zn deficiency are poorly understood. Using global proteomic analysis, we identified two proteins (ZCRP-A/B, Zn/Co Responsive Protein A/B) among four diatom species that became abundant under Zn/Co limitation. Characterization using reverse genetic techniques and homology data suggests putative Zn/Co chaperone and membrane-bound transport complex component roles for ZCRP-A (a COG0523 domain protein) and ZCRP-B, respectively. Metaproteomic detection of ZCRPs along a Pacific Ocean transect revealed increased abundances at the surface (<200 m) where dZn and dCo were scarcest, implying Zn nutritional stress in marine algae is more prevalent than previously recognized. These results demonstrate multiple adaptive responses to Zn scarcity in marine diatoms that are deployed in low Zn regions of the Pacific Ocean. Here the authors identify two zinc/cobalt responsive proteins (ZCRPs) in marine diatoms, determining their functional roles in trace metal transport and homeostasis, as well as their application as an indicator of oceanic Zn stress.
登录
查看更多内容
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
2.2
作者：
Bartual, Ana;Angel Galvez, J.;Ojeda, Fernando
通讯作者：
Ojeda, Fernando
影响因子：
11
作者：
Jensen, Erik L.;Clement, Romain;Gontero, Brigitte
通讯作者：
Gontero, Brigitte
影响因子：
4.5
作者：
BRULAND, KW
通讯作者：
BRULAND, KW