Detection and characterization of Epstein-Barr virus in clinical specimens.
Detection and characterization of Epstein-Barr virus in clinical specimens.
复制标题
临床标本中 Epstein-Barr 病毒的检测和表征。
作者：
R. Ambinder;R. Mann
EB 病毒 (EBV) 与多种良性和恶性疾病有关。最近添加到该列表中的包括口腔毛状白斑；霍奇金淋巴瘤的一个子集，特别是那些具有混合细胞组织学或发生在不发达国家的肿瘤；免疫功能低下的弥漫性大细胞/免疫母细胞淋巴瘤的一个亚型，特别是原发性中枢神经系统淋巴瘤；外周 T 细胞淋巴瘤的一个亚型；以及胃癌的一个子集，特别是未分化癌。该病毒的生物学有几个独特的方面对于临床标本中的病毒研究非常重要。基因组中重复元件的存在有助于通过各种杂交技术检测病毒核酸以及表征病毒感染组织的克隆性。潜伏病毒感染与受感染细胞中病毒基因表达的几种不同模式有关。潜在基因产物很重要，因为它们具有生长调节和转化特性以及它们引发的有效细胞毒性 T 细胞反应。 EBER RNA 转录本的丰富表达使得敏感检测 EBV 相关肿瘤中的潜在感染成为可能。抗病毒核苷类似物可以抑制裂解感染。两种裂解基因产物因其与细胞蛋白 BCL-2 和 IL-10 的同源性而受到特别关注。最近已对两种在淋巴细胞永生化和转化方面具有不同特性的病毒生物型或毒株进行了表征。目前的证据表明不同的生物型与特定的恶性肿瘤相关。 EBV 与各种疾病过程之间关系的表征对于诊断和治疗以及更好地了解这些糖尿病的流行病学和发病机制具有重要意义。
Epstein-Barr virus (EBV) is associated with a wide spectrum of benign and malignant diseases. Recent additions to the list include oral hairy leukoplakia; a subset of Hodgkin's lymphomas, particularly those with mixed cellularity histology or those occurring in underdeveloped countries; a subset of diffuse large cell/immunoblastic lymphoma in the immunocompromised, particularly primary central nervous system lymphoma; a subset of peripheral T cell lymphomas; and a subset of gastric carcinomas, particularly undifferentiated carcinomas. There are several distinctive aspects of the biology of the virus that are important in investigations of virus in clinical specimens. The presence of repeated elements in the genome facilitates detection of viral nucleic acids by a variety of hybridization techniques as well as the characterization of the clonality of virus-infected tissues. Latent viral infection is associated with several different patterns of viral gene expression in infected cells. Latent gene products are important because of their growth-regulating and -transforming properties as well as the potent cytotoxic T cell response they elicit. The abundant expression of the EBER RNA transcripts makes possible the sensitive detection of latent infection in EBV-associated tumors. Lytic infection can be inhibited by antiviral nucleoside analogues. Two lytic gene products are of special interest because of their homology to the cellular proteins BCL-2 and interleukin-10. Two viral biotypes or strains with different properties in terms of lymphocyte immortalization and transformation have recently been characterized. Current evidence suggests a differential biotype association with particular malignancies. Characterization of the association of EBV with various disease processes promises to be important for diagnosis and treatment as well as for a better understanding of the epidemiology and pathogenesis of these diabetes.
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影响因子：
56.9
作者：
J. Sixbey;Q. Yao
通讯作者：
J. Sixbey;Q. Yao
DOI：
--
发表时间：
1991-06
期刊：
The American journal of pathology
影响因子：
--
作者：
Tzyy Choou Wu;Risa B. Mann;Jonathan I. Epstein;E. Macmahon;W. Lee;Patricia Charache;S. Hayward;Robert J. Kurman;Gary S. Hayward;R. Ambinder
通讯作者：
Tzyy Choou Wu;Risa B. Mann;Jonathan I. Epstein;E. Macmahon;W. Lee;Patricia Charache;S. Hayward;Robert J. Kurman;Gary S. Hayward;R. Ambinder
霍奇金病中 Epstein-Barr 病毒 (EBV) 的亚型：B 型 EBV 与免疫功能低下之间的关联。
影响因子：
20.3
作者：
Boyle,MJ;Vasak,E;Tschuchnigg,M;Turner,JJ;Sculley,T;Penny,R;Cooper,DA;Tindall,B;Sewell,WA
通讯作者：
Sewell,WA
艾滋病相关淋巴瘤中 c-myc 癌基因频繁激活，而 Epstein-Barr 病毒基因组很少出现。
影响因子：
20.3
作者：
Subar,M;Neri,A;Inghirami,G;Knowles,DM;Dalla-Favera,R
通讯作者：
Dalla-Favera,R
快速原位杂交用于诊断潜伏的 Epstein-Barr 病毒感染。
影响因子：
3.3
作者：
Barletta,JM;Kingma,DW;Ling,Y;Charache,P;Mann,RB;Ambinder,RF
通讯作者：
Ambinder,RF