Characterization of Two Novel Bacillus thuringiensis Cry8 Toxins Reveal Differential Specificity of Protoxins or Activated Toxins against Chrysomeloidea Coleopteran Superfamily.
Characterization of Two Novel Bacillus thuringiensis Cry8 Toxins Reveal Differential Specificity of Protoxins or Activated Toxins against Chrysomeloidea Coleopteran Superfamily.
复制标题
两种新型苏云金芽孢杆菌 Cry8 毒素的表征揭示了原毒素或活化毒素针对叶鞘鞘翅目超家族的差异特异性
DOI：
10.3390/toxins12100642
复制
发表时间：
2020-10-05
期刊：
影响因子：
4.2
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Shu C;Yan G;Huang S;Geng Y;Soberón M;Bravo A;Geng L;Zhang J
Scarabaeoidea and Chrysomeloidea insects are agriculture-destructive coleopteran pests. Few effective Bacillus thuringiensis (Bt) insecticidal proteins against these species have been described. Bt isolate BtSU4 was found to be active against coleopteran insects. Genome sequencing revealed two new cry8 genes in BtSU4, designated as cry8Ha1 and cry8Ia1. Both genes expressed a 135 kDa protoxin forming irregular shape crystals. Bioassays performed with Cry8Ha1 protoxin showed that it was toxic to both larvae and adult stages of Holotrichia parallela, also to Holotrichia oblita adults and to Anoplophora glabripennis larvae, but was not toxic to larval stages of H. oblita or Colaphellus bowringi. The Cry8Ia1 protoxin only showed toxicity against H. parallela larvae. After activation with chymotrypsin, the Cry8Ha1 activated toxin lost its insecticidal activity against H. oblita adults and reduced its activity on H. parallela adults, but gained toxicity against C. bowringi larvae, a Chrysomeloidea insect pest that feeds on crucifer crops. The chymotrypsin activated Cry8Ia1 toxin did not show toxicity to any one of these insects. These data show that Cry8Ha1 and Cry8Ia1 protoxin and activated toxin proteins have differential toxicity to diverse coleopteran species, and that protoxin is a more robust protein for the control of coleopteran insects.
登录
查看更多内容
影响因子：
1.3
作者：
Bouchard P;Bousquet Y;Davies AE;Alonso-Zarazaga MA;Lawrence JF;Lyal CH;Newton AF;Reid CA;Schmitt M;Slipiński SA;Smith AB
通讯作者：
Smith AB
Bt 蛋白的双重作用模式：原毒素对抗抗性昆虫的功效
影响因子：
4.6
作者：
Tabashnik BE;Zhang M;Fabrick JA;Wu Y;Gao M;Huang F;Wei J;Zhang J;Yelich A;Unnithan GC;Bravo A;Soberón M;Carrière Y;Li X
通讯作者：
Li X
影响因子：
5
作者：
Chen, J;Dai, LY;Lu, MZ
通讯作者：
Lu, MZ
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
3.8
作者：
Bravo, Alejandra;Likitvivatanavong, Supaporn;Gill, Sarjeet S.;Soberon, Mario
通讯作者：
Soberon, Mario