Slug expression is an independent prognostic parameter for poor survival in colorectal carcinoma patients.
Slug expression is an independent prognostic parameter for poor survival in colorectal carcinoma patients.
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DOI：
10.1038/sj.bjc.6603193
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发表时间：
2006-06-19
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
Slug是Snail转录因子家族的成员之一，通过抑制包括E-cadherin在内的多种上皮标志物和粘附分子，在上皮-间质转化（EMT）的调控中起着至关重要的作用。近年来，有研究报道Slug在乳腺癌、食管癌中表达，并伴有生存期缩短。在这项研究中，我们首先研究了Slug mRNA的表达在五个结直肠癌细胞系的逆转录-聚合酶链反应。此外，我们研究了Slug和E-cadherin表达免疫组化在138例大肠癌。Slug mRNA在5个大肠癌细胞系中的4个中有明确表达。Slug和E-cadherin的阳性表达率分别为37%和58%。Slug蛋白的阳性表达与Dukes分期、远处转移有关（P=0.0027和0.0007），Slug蛋白的阳性表达对患者的总生存期有显著影响（P<0.0001，log-rank检验）。Slug阳性表达和E-cadherin表达降低的患者预后最差（P<0.0001，log-rank检验）。多因素分析显示Slug表达是独立的预后因素。提示Slug蛋白在大肠癌中的阳性表达可作为判断预后的一个重要指标。
Slug, a member of the Snail family of transcription factors, plays a crucial role in the regulation of epithelial-mesenchymal transition (EMT) by suppressing several epithelial markers and adhesion molecules including E-cadherin. Recently, several studies have reported Slug to be expressed in breast carcinoma, oesophageal carcinoma accompanied with shorter survival. In this study, we first investigated expression of Slug mRNA in five colorectal carcinoma cell lines by reverse transcription–polymerase chain reaction. Furthermore, we investigated Slug and E-cadherin expression by immunohistochemistry in 138 patients with colorectal carcinoma. Slug mRNA was clearly expressed in four out of five colorectal carcinoma cell lines. Positive expression of Slug and E-cadherin was observed in 37 and 58% of cases, respectively. The positive expression of Slug was significantly associated with Dukes stage and distant metastasis (P=0.0027 and 0.0007), and the positive expression of Slug had a significant impact on patient overall survival (P<0.0001, log-rank test). Moreover, patients with positive expression of Slug and reduced expression of E-cadherin showed the worst prognosis (P<0.0001, log-rank test). Multivariate analysis indicated that Slug expression was an independent prognostic factor. These results suggest that positive Slug expression in colorectal carcinoma patients may become a significant parameter of poor prognosis.
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