Neuromodulation Effect of Very Low Intensity Transcranial Ultrasound Stimulation on Multiple Nuclei in Rat Brain.
Neuromodulation Effect of Very Low Intensity Transcranial Ultrasound Stimulation on Multiple Nuclei in Rat Brain.
复制标题
极低强度经颅超声刺激对大鼠脑内多核的神经调节作用。
DOI：
10.3389/fnagi.2021.656430
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发表时间：
2021
影响因子：
4.8
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Liu Y;Wang G;Cao C;Zhang G;Tanzi EB;Zhang Y;Zhou W;Li Y
低强度经颅超声刺激（TUS）是一种非侵入性的神经调节技术，具有高空间分辨率和可行的穿透深度。迄今为止，TUS调制神经振荡的机制尚未完全了解。本研究设计了一种非常低的声强（AI）TUS系统，与以前用于测量不同TUS参数下的局部神经振荡模式的方法相比，该系统产生的AI超声脉冲（ISPTA < 0.5 W/cm 2）显著减少。在三组模拟参数下，记录了五个脑核团的TUS局部场电位（LFP）。谱估计，时频分析（TFA），相对功率分析方法已被应用于研究不同的刺激参数下的神经振荡模式。在PRF、500 Hz和1 kHz TUS下，当ISPTA超过12 mW/cm 2时，在选定的核团中出现具有自动节律模式的高幅值LFP活动。使用TFA，高频能量（慢伽马和高伽马）在自动节律模式期间显著增加。我们观察到一个初始平台时，核反应ISPTA达到16.4 mW/cm 2的RPF 500 Hz和20.8 mW/cm 2的RPF 1 kHz。反应核数开始减少，而ISPTA继续增加。在1.5 kHz TUS下，未观察到自动节律模式，但TUS期间慢频率功率增加。在RPF = 1.5kHz，ISPTA = 8.8mW/cm ~ 2时，TUS抑制大部分频段，产生明显的慢波（θ和δ频段）。这些结果表明，极低强度经颅超声刺激（VLTUS）在大鼠模型中的特定参数下发挥显着的神经调质作用，并可能是一个有效的工具，研究神经元生理学。
Low-intensity transcranial ultrasound stimulation (TUS) is a non-invasive neuromodulation technique with high spatial resolution and feasible penetration depth. To date, the mechanisms of TUS modulated neural oscillations are not fully understood. This study designed a very low acoustic intensity (AI) TUS system that produces considerably reduced AI Ultrasound pulses (ISPTA < 0.5 W/cm2) when compared to previous methods used to measure regional neural oscillation patterns under different TUS parameters. We recorded the local field potential (LFP) of five brain nuclei under TUS with three groups of simulating parameters. Spectrum estimation, time-frequency analysis (TFA), and relative power analysis methods have been applied to investigate neural oscillation patterns under different stimulation parameters. Under PRF, 500 Hz and 1 kHz TUS, high-amplitude LFP activity with the auto-rhythmic pattern appeared in selected nuclei when ISPTA exceeded 12 mW/cm2. With TFA, high-frequency energy (slow gamma and high gamma) was significantly increased during the auto-rhythmic patterns. We observed an initial plateau in nuclei response when ISPTA reached 16.4 mW/cm2 for RPF 500 Hz and 20.8 mW/cm2 for RPF 1 kHz. The number of responding nuclei started decreasing while ISPTA continued increasing. Under 1.5 kHz TUS, no auto-rhythmic patterns have been observed, but slow frequency power was increased during TUS. TUS inhibited most of the frequency band and generated obvious slow waves (theta and delta band) when stimulated at RPF = 1.5 kHz, ISPTA = 8.8 mW/cm2. These results demonstrate that very low intensity Transcranial Ultrasound Stimulation (VLTUS) exerts significant neuromodulator effects under specific parameters in rat models and may be a valid tool to study neuronal physiology.
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DOI：
10.1073/pnas.1716933115
发表时间：
2018-03-27
影响因子：
11.1
作者：
Roach JP;Pidde A;Katz E;Wu J;Ognjanovski N;Aton SJ;Zochowski MR
通讯作者：
Zochowski MR
影响因子：
16.2
作者：
Guo, Hongsun;Hamilton, Mark, II;Lim, Hubert H.
通讯作者：
Lim, Hubert H.
影响因子：
7.7
作者：
Perera T;George MS;Grammer G;Janicak PG;Pascual-Leone A;Wirecki TS
通讯作者：
Wirecki TS
影响因子：
2.6
作者：
Hakimova, Hilola;Kim, Sangwoo;Jeon, Daejong
通讯作者：
Jeon, Daejong
影响因子：
2.5
作者：
Henrie, JA;Shapley, R
通讯作者：
Shapley, R