Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.
Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.
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DOI：
10.1038/ncomms12567
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发表时间：
2016-08-26
影响因子：
16.6
通讯作者：
Kitagawa, Daiju
中科院分区：
文献类型：
--
作者：
Tsuchiya, Yuki;Yoshiba, Satoko;Gupta, Akshari;Watanabe, Koki;Kitagawa, Daiju
中心粒被着丝点周围物质(PCM)包围，是中心体的核心结构。新形成的女儿中心粒成长为具有功能的母中心粒。然而，人们对其潜在机制仍知之甚少。在这里，我们展示了Cep295，一种进化上保守的蛋白质，是产生一个真正的母中心粒组织功能中心体所必需的。我们发现，在原着丝粒组装的早期阶段，Cep295被募集到近端的中心粒壁上。然后，Cep295充当适当组装女儿中心粒的支架。我们还发现Cep295直接结合并将Cep192招募到子代中心粒上，这可能赋予了新的母体中心粒对PCM组装、微管组织中心活动和中心粒形成的功能。这些发现导致我们提出Cep295作用于中心粒形成的保守途径的上游，并促进子代向母体的中心粒转化。新形成的中心粒成熟为具有功能的母中心粒。在这里，作者证明了支架蛋白Cep295被招募到前着丝粒组装位置，并帮助女儿中心粒组装和女儿到母亲的中心粒转化。
Centrioles surrounded by pericentriolar material (PCM) serve as the core structure of the centrosome. A newly formed daughter centriole grows into a functional mother centriole. However, the underlying mechanisms remain poorly understood. Here we show that Cep295, an evolutionarily conserved protein, is required for generation of a bona fide mother centriole organizing a functional centrosome. We find that Cep295 is recruited to the proximal centriole wall in the early stages of procentriole assembly. Cep295 then acts as a scaffold for the proper assembly of the daughter centriole. We also find that Cep295 binds directly to and recruits Cep192 onto the daughter centriole wall, which presumably endows the function of the new mother centriole for PCM assembly, microtubule-organizing centre activity and the ability for centriole formation. These findings led us to propose that Cep295 acts upstream of the conserved pathway for centriole formation and promotes the daughter-to-mother centriole conversion. Newly formed centrioles mature into functional mother centrioles. Here the authors show that the scaffolding protein Cep295 is recruited to the procentriole assembly site and assists daughter centriole assembly and daughter-to-mother centriole conversion.
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影响因子：
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