DIFFERENTIATED CORTICOSTEROID PRODUCTION AND REGENERATION AFTER SELECTIVE TRANSPLANTATION OF CULTURED AND NONCULTURED ADRENOCORTICAL CELLS IN THE ADRENALECTOMIZED RAT1
DIFFERENTIATED CORTICOSTEROID PRODUCTION AND REGENERATION AFTER SELECTIVE TRANSPLANTATION OF CULTURED AND NONCULTURED ADRENOCORTICAL CELLS IN THE ADRENALECTOMIZED RAT1
复制标题
肾上腺切除大鼠选择性移植培养和非培养肾上腺皮质细胞后分化的皮质类固醇产生和再生
DOI：
10.1097/00007890-200009150-00022
复制
发表时间：
2000
期刊：
影响因子：
6.2
通讯作者：
G. Scheumann
中科院分区：
文献类型：
--
作者：
O. Teebken;G. Scheumann
在刘易斯大鼠双侧肾上腺切除术后，研究同基因肾上腺细胞移植对分化激素分泌和皮质再生的影响。方法.通过密度梯度分离获得球状细胞（密度1.061±0.001 g/ml）和束状细胞（密度1.034±0.003 g/ml）的纯化细胞悬液，并立即或在29天培养期后移植到肾被膜下。在移植培养的肾小球（CG-Tx）或培养的束状体细胞（CF-Tx）、非培养的肾小球细胞（G-Tx）或非培养的束状体细胞（F-Tx）或两种细胞类型（GF-Tx）后，在30、120和360天后处死动物用于形态学研究。在移植后第3、30、120和360天抽取血浆样本用于测量皮质酮和醛固酮以及24小时尿钠和钾水平。结果在原代培养束状细胞数量保持稳定，但球状细胞数量增加到近10倍。通过组织化学在每个移植物中显示了活的皮质细胞，但只有G-Tx，CG-Tx和GF-Tx组（纯化的球状珊瑚和束状珊瑚细胞悬液）显示了新皮质样结构。我们发现血浆（尿）皮质酮从未治疗动物的术前256-304 ng/ml（226-239 ng/天）降低到移植后3天的一半水平，在治疗后30天所有研究组均升高至正常值（数据以范围表示）。血浆醛固酮浓度，150-180 pg/ml的未处理的大鼠，下降到无法检测的水平，为1周后双侧肾上腺切除术。在第30天，组GF-Tx、G-Tx和CG-Tx显示相当的醛固酮血浆浓度（104-122 pg/ml）;然而，F-Tx和CF-Tx中的水平为19-49 pg/ml，并且在观察期内没有显著增加。结论.来自小球的细胞保持活力，产生醛固酮和皮质酮，并再生新皮质，其细胞在组织学上类似小球和束状细胞。因此，它们适合于肾上腺皮质移植。与此相反，来自束状体的细胞保持活力，但不再生球状体，也不产生醛固酮。这些结果表明，细胞迁移模型，其中肾小球细胞可以获得束状细胞的表型，因为它们可以向心性迁移，更可能是肾上腺皮质带状化的正确解释。
Syngeneic transplantation of adrenocytes was investigated in Lewis rats in regard to differentiated hormone secretion and cortex regeneration after bilateral adrenalectomy as an alternative to steroid substitution. Methods. Purified cell suspensions of glomerulosa (density 1.061±0.001 g/ml) and fasciculata (density 1.034±0.003 g/ml) cells were obtained by density gradient separation and were transplanted under the kidney capsule either immediately or after a 29-day culture period. Animals were killed after transplantation of cultured glomerulosa (CG-Tx) or cultured fasciculata cells (CF-Tx), noncultured glomerulosa cells (G-Tx) or non-cultured fasciculata cells (F-Tx), or both cell types (GF-Tx) for morphological studies after 30, 120, and 360 days. Plasma samples were drawn for measurement of corticosterone and aldosterone as well as 24 hr-urine for sodium and potassium levels at day 3, 30, 120, and 360 after transplantation. Results. In primary culture fasciculata cell number remained stationary although glomerulosa cell number increased to almost 10-fold. Vital cortex cells were demonstrated in each explanted graft by histochemistry but only group G-Tx, CG-Tx, and GF-Tx (purified cell suspensions of zona glomerulosa and fasciculata) showed neocortex-like structures. We found plasma (urine) corticosterone to decrease from preoperatively 256–304 ng/ml (226–239 ng/day) in untreated animals to levels about half as high 3 days after transplantation, increasing to normal values in all study groups 30 days after treatment (data given as range). Plasma aldosterone concentrations, 150–180 pg/ml in untreated rats, decreased to nondetectable levels for 1 week after bilateral adrenalectomy. At day 30 group GF-Tx, G-Tx, and CG-Tx showed comparable aldosterone plasma concentrations (104–122 pg/ml); however, levels in F-Tx and CF-Tx were 19–49 pg/ml, and did not increase significantly within the observation period. Conclusions. Cells derived from the zona glomerulosa maintain viability, produce both aldosterone and corticosterone, and regenerate a neocortex with cells that histologically resemble both zona glomerulosa and fasciculata cells. They are therefor suitable for adrenocortical transplantation. In contrast, cells derived from the zona fasciculata maintain viability, but do not regenerate zona glomerulosa and do not produce aldosterone. These results suggest that the cell migration model, in which zona glomerulosa cells can acquire the phenotype of zona fasciculata cells as they can migrate centripetaly, is more likely the correct explanation of adrenocortical zonation.
登录
查看更多内容
体外培养和环孢素 A 处理对同种异体肾上腺髓质移植物存活的影响。
DOI：
10.1016/0022-4804(89)90042-5
发表时间：
1989
期刊：
The Journal of surgical research
影响因子：
--
作者：
Ricordi,C;Cryer,PE;Lacy,PE
通讯作者：
Lacy,PE
影响因子：
4.8
作者：
C. Wotus;B. Levay-Young;L. Rogers;C. Gomez-Sanchez;W. Engeland
通讯作者：
C. Wotus;B. Levay-Young;L. Rogers;C. Gomez-Sanchez;W. Engeland
细胞移植：研究肾上腺皮质细胞生物学、生理学和衰老的工具。
影响因子：
2.1
作者：
Hornsby,PJ;Thomas,M;Northrup,SR;Popnikolov,NK;Wang,X;Tunstead,JR;Zheng,J
通讯作者：
Zheng,J
钾刺激的血管紧张素从灌注的肾上腺囊和酶促分散的肾小球带细胞中释放。
影响因子：
4.8
作者：
Kifor,I;Moore,TJ;Fallo,F;Sperling,E;Chiou,CY;Menachery,A;Williams,GH
通讯作者：
Williams,GH
再生早期阶段肾上腺皮质细胞的分化和增殖。
DOI：
--
发表时间：
1985
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Taki,TM;Nickerson,PA
通讯作者：
Nickerson,PA