Leucine repeat adaptor protein 1 interacts with Dishevelled to regulate gastrulation cell movements in zebrafish.
Leucine repeat adaptor protein 1 interacts with Dishevelled to regulate gastrulation cell movements in zebrafish.
复制标题
亮氨酸重复接头蛋白 1 与 Disheveled 相互作用调节斑马鱼原肠胚细胞运动
DOI：
10.1038/s41467-017-01552-x
复制
发表时间：
2017-11-07
影响因子：
16.6
通讯作者：
Shi DL
中科院分区：
文献类型：
--
作者：
Cheng XN;Shao M;Li JT;Wang YF;Qi J;Xu ZG;Shi DL
原肠形成是一个基本的形态发生事件，需要极化的细胞行为来协调不对称的细胞运动。Wnt/PCP信号在这一过程中起着关键作用。disheveled是一种重要的保守支架蛋白，它将Wnt/PCP信号从膜受体传递到细胞骨架组织的调节。然而，目前尚不清楚其活性是如何调节下游效应物的激活的。在这里，我们报道了Lurap1是一种无序相互作用蛋白，在脊椎动物原肠形成过程中调节Wnt/PCP信号的收敛和伸展运动。它的功能丧失导致散乱膜定位增强和JNK活性增加。在母合子clurap1突变的斑马鱼胚胎中，细胞极性和定向运动被破坏。延时分析表明，Lurap1、disheveled和JNK在功能上相互作用，协调极化细胞的突出活动，并且Lurap1是运动细胞中中心粒/MTOC协调定位所必需的。这些发现表明，Lurap1在原肠胚运动过程中调节细胞极化和运动行为。
Gastrulation is a fundamental morphogenetic event that requires polarised cell behaviours for coordinated asymmetric cell movements. Wnt/PCP signalling plays a critical role in this process. Dishevelled is an important conserved scaffold protein that relays Wnt/PCP signals from membrane receptors to the modulation of cytoskeleton organisation. However, it remains unclear how its activity is regulated for the activation of downstream effectors. Here, we report that Lurap1 is a Dishevelled-interacting protein that regulates Wnt/PCP signalling in convergence and extension movements during vertebrate gastrulation. Its loss-of-function leads to enhanced Dishevelled membrane localisation and increased JNK activity. In maternal-zygoticlurap1mutant zebrafish embryos, cell polarity and directional movement are disrupted. Time-lapse analyses indicate that Lurap1, Dishevelled, and JNK functionally interact to orchestrate polarised cellular protrusive activity, and Lurap1 is required for coordinated centriole/MTOC positioning in movement cells. These findings demonstrate that Lurap1 functions to regulate cellular polarisation and motile behaviours during gastrulation movements.
登录
查看更多内容
影响因子：
10.5
作者：
Habas, R;Dawid, IB;He, X
通讯作者：
He, X
影响因子：
16.6
作者：
Carvajal-Gonzalez JM;Roman AC;Mlodzik M
通讯作者：
Mlodzik M
影响因子：
4.6
作者：
Carreira-Barbosa, F;Concha, ML;Tada, M
通讯作者：
Tada, M
影响因子：
11.8
作者：
Cheyette, BNR;Waxman, JS;Moon, RT
通讯作者：
Moon, RT
影响因子：
11.8
作者：
Gray, Ryan S.;Roszko, Isabelle;Solnica-Krezel, Lilianna
通讯作者：
Solnica-Krezel, Lilianna