Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase.

Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase.
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58 千道尔顿人中性粒细胞胶原酶的表征:与人成纤维细胞胶原酶的比较。

DOI:
10.1021/bi00499a008
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发表时间:
1990
期刊:
影响因子:
2.9
通讯作者:
VanWart,HE
VanWart,HE
中科院分区:
生物学3区
文献类型:
--
作者:
Mallya,SK;Mookhtiar,KA;Gao,Y;Brew,K;Dioszegi,M;Birkedal-Hansen,H;VanWart,HE

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材料与方法大鼠型胶原蛋白来源于尾腱,牛型胶原蛋白来源于鼻中隔,人型胶原蛋白来源于胎盘。1缩写:HNC,人中性粒细胞胶原酶;HNG,人中性粒细胞明胶酶(92-kDa IV型胶原酶);HFC,人成纤维细胞胶原酶;HFS,人成纤维细胞基质溶素;HFG,人成纤维细胞明胶酶(72-kDa IV型胶原酶);PUMP-1,推定的金属蛋白酶1;MMP matrixmetalloproteinase;PCMB, / ?——(chloromercuri)苯甲酸酯;APMA,对氨基苯基醋酸汞;PMSF,苯甲磺酰氟;SDS-PAGE,十二烷基硫酸钠聚丙烯酰胺凝胶电泳;Fmoc 9-fluorenylmethoxycarbonyl;三辛,jV-[三(羟甲基)甲基]甘氨酸;三,三羟基甲基)氨基甲烷;Mes, 2-(/V-morpholino)乙磺酸;聚氧乙烯(23)十二烷基醚Brij-35;乙二胺四乙酸;Asr specificactivity。
Materials and Methods Materials. Rat type Icollagen was isolated from tail tendon, bovine type II from nasal septum, and human types I and III from placenta. These collagens were extracted and purified, 1 Abbreviations: HNC, human neutrophil collagenase; HNG, human neutrophil gelatinase (92-kDa type IV collagenase); HFC, human fi-broblast collagenase; HFS, human fibroblast stromelysin; HFG, human fibroblast gelatinase (72-kDa type IV collagenase); PUMP-1, putative metalloproteinase 1; MMP, matrixmetalloproteinase; PCMB,/?-(chloromercuri) benzoate; APMA, p-aminophenylmercuric acetate; PMSF, phenylmethanesulfonyl fluoride; SDS-PAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; Fmoc, 9-fluorenylmethoxycarbonyl; Tricine, jV-[tris (hydroxymethyl) methyl] glycine; Tris, trisfhydroxy-methyl) aminomethane; Mes, 2-(/V-morpholino) ethanesulfonic acid; Brij-35, polyoxyethylene (23) lauryl ether; EDTA, ethylenediaminetetraacetic acid; Asr, specificactivity.
DOI: 10.1016/s0021-9258(17)44166-4
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
G. Stricklin;H. Welgus
通讯作者: H. Welgus
通过细菌和组织胶原酶对可溶性 I、II 和 III 型 3H 乙酰化胶原进行水解的准确定量测定。
DOI: 10.1016/0003-2697(86)90558-0
发表时间: 1986
影响因子: 2.9
作者:
Mallya,SK;Mookhtiar,KA;VanWart,HE
通讯作者: VanWart,HE
DOI: 10.1016/0076-6879(87)44177-3
发表时间: 1987
影响因子: --
作者:
H. Birkedal‐Hansen
通讯作者: H. Birkedal‐Hansen
DOI: --
发表时间: 1987
期刊: The Journal of biological chemistry
影响因子: --
作者:
Fields,GB;VanWart,HE;Birkedal-Hansen,H
通讯作者: Birkedal-Hansen,H
人皮肤成纤维细胞前胶原酶:有机汞和胰蛋白酶的激活机制。
DOI: 10.1021/bi00270a009
发表时间: 1983
期刊: Biochemistry
影响因子: 2.9
作者:
Stricklin,GP;Jeffrey,JJ;Roswit,WT;Eisen,AZ
通讯作者: Eisen,AZ