The distribution of normal and male-sterile cytoplasms in Chinese sugar-beet germplasm

The distribution of normal and male-sterile cytoplasms in Chinese sugar-beet germplasm
复制标题

中国甜菜种质中正常和雄性不育细胞质的分布

DOI:
10.1007/s10681-008-9796-0
复制
发表时间:
2008
期刊:
影响因子:
1.9
通讯作者:
Kitazaki, Kazuyoshi
Kitazaki, Kazuyoshi
中科院分区:
农林科学3区
文献类型:
--
作者:
Xu, Dechang;Mikami, Tetsuo;Cheng, Dayou;Kubo, Tomohiko;Kitazaki, Kazuyoshi

文献摘要

参考文献

相似文献

利用叶绿体petG-psbE区和线粒体小卫星基因座的多态性,对42个中国甜菜育种系进行了正常和雄性不育(Owen)细胞质多态性的鉴定。检测到的多态性允许在整个样品中区分三种细胞质类型,一种与Owen细胞质相关,第二种与保持系近交系'TK-81 mm-O'型细胞质(称为正常-1细胞质)相关,第三种与另一种保持系近交系'NK-310 mm-O'型细胞质(正常-2细胞质)相关。进行Western印迹分析,以确认表达的雄性不育相关蛋白(前SATP 6)发生在植物欧文细胞质,但不是在植物中的正常-1或正常-2细胞质。在42个育种系的检查,14个完全正常(正常-1和/或正常-2)细胞质和11个只有欧文细胞质。其余17个系具有正常和Owen细胞学,并且值得注意的是,这17个系中的一些系已被期望成为上级保持系的来源。因此,结果表明,细胞质的分子鉴定是必要的,以避免浪费资源,由于试图开发的保持基因型从植物欧文细胞质。
Forty-two Chinese sugar-beet breeding lines were evaluated for the presence of normal and male-sterile (Owen) cytoplasms using polymorphisms in the chloroplastpetG-psbEregion as well as in the mitochondrial minisatellite loci. The polymorphisms detected allowed the distinction of three cytoplasm types over the whole sample, one being associated with Owen cytoplasm, a second with the maintainer inbred ‘TK-81mm-O’-type cytoplasm (termed normal-1 cytoplasm) and a third with another maintainer inbred ‘NK-310mm-O’-type cytoplasm (normal-2 cytoplasm). Western blot analysis was carried out to confirm that expression of the male-sterility-associated protein (preSATP6) occurred in plants with Owen cytoplasm but not in plants with either normal-1 or normal-2 cytoplasm. Of the 42 breeding lines examined, 14 had exclusively normal (normal-1 and/or normal-2) cytoplasm and 11 had only Owen cytoplasm. The remaining 17 lines possessed both normal and Owen cytoplasms, and noticeably, some of these 17 lines have been expected to become the source of superior maintainer lines. The results thus show that molecular identification of the cytoplasm is required to avoid wasting resources on account of attempting to develop the maintainer genotype from plants with Owen cytoplasm.
DOI: 10.1007/978-94-009-3137-4_2
发表时间: 1986
期刊: --
影响因子: --
作者:
M. A. Stevens;C. Rick
通讯作者: M. A. Stevens;C. Rick
DOI: 10.2135/cropsci1965.0011183x000500050009x
发表时间: 1965-01-01
期刊: CROP SCI
影响因子: 2.3
作者:
BLISS, FREDRICK A.;GABELMAN, W. H.
通讯作者: GABELMAN, W. H.
DOI: 10.2135/cropsci2002.0540
发表时间: 2002-03-01
期刊: CROP SCIENCE
影响因子: 2.3
作者:
Hjerdin-Panagopoulos, A;Kraft, T;Nilsson, NO
通讯作者: Nilsson, NO
DOI: 10.1111/j.1439-0523.1993.tb00641.x
发表时间: 1993-12-01
期刊: PLANT BREEDING
影响因子: 2
作者:
PILLEN, K;STEINRUCKEN, G;JUNG, C
通讯作者: JUNG, C
DOI: 10.5274/jsbr.15.6.538
发表时间: 1969-07
期刊: Journal of Sugarbeet Research
影响因子: --
作者:
J. Theurer;G. K. Ryser
通讯作者: J. Theurer;G. K. Ryser