A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis.
A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis.
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DOI：
10.1083/jcb.201204085
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发表时间：
2012-07-23
期刊：
影响因子：
--
通讯作者：
Watanabe Y
中科院分区：
文献类型：
--
作者：
Morimoto A;Shibuya H;Zhu X;Kim J;Ishiguro K;Han M;Watanabe Y
哺乳动物减数分裂特异性KASH蛋白KASH 5将端粒相关的SUN 1蛋白连接到减数分裂染色体运动中涉及的细胞质力产生机制。在酵母和蠕虫中，KASH（Klarsicht/ANC-1/Syne/homology）结构域和SUN（Sad-1/SAND-84）结构域核被膜（NE）蛋白在减数分裂染色体运动和同源配对中起着至关重要的作用。然而，尽管脊椎动物SUN结构域蛋白SUN 1参与这些过程，但其伴侣仍被鉴定。基于小鼠精母细胞亚细胞定位筛选，我们确定了一种新的生殖细胞特异性蛋白，KASH 5，专门定位于精母细胞和卵母细胞从细线期到双线期的端粒。KASH 5具有迄今未知的KASH相关序列，其直接与SUN 1相互作用并介导端粒定位。因此，KASH 5是哺乳动物减数分裂特异性KASH结构域蛋白。我们发现，减数分裂染色体运动依赖于微管和KASH 5相互作用的微管相关的动力蛋白-动力蛋白复合物。这些结果表明，KASH 5连接端粒相关的SUN 1蛋白质的细胞质力产生机制参与减数分裂染色体运动。我们的研究表明，由SUN-KASH NE桥介导的减数分裂同源配对机制在真核生物中是高度保守的。
The mammalian meiosis-specific KASH protein KASH5 connects the telomere-associated SUN1 protein to the cytoplasmic force–generating mechanism involved in meiotic chromosome movement. In yeasts and worms, KASH (Klarsicht/ANC-1/Syne/homology) domain and SUN (Sad-1/UNC-84) domain nuclear envelope (NE) proteins play a crucial role in meiotic chromosome movement and homologue pairing. However, although the vertebrate SUN domain protein SUN1 is involved in these processes, its partner has remained identified. Based on subcellular localization screening in mouse spermatocytes, we identified a novel germ cell–specific protein, KASH5, that localized exclusively at telomeres from the leptotene to diplotene stages in both spermatocytes and oocytes. KASH5 possesses hitherto unknown KASH-related sequences that directly interacted with SUN1 and mediated telomere localization. Thus, KASH5 is a mammalian meiosis-specific KASH domain protein. We show that meiotic chromosome movement depended on microtubules and that KASH5 interacted with the microtubule-associated dynein–dynactin complex. These results suggest that KASH5 connects the telomere-associated SUN1 protein to the cytoplasmic force–generating mechanism involved in meiotic chromosome movement. Our study strongly suggests that the meiotic homologue-pairing mechanism mediated by the SUN–KASH NE bridge is highly conserved among eukaryotes.
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在发育，减数分裂和疾病期间，太阳结构域和kash域蛋白质。
DOI：
10.1007/s00018-008-8713-y
发表时间：
2009-05
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Fridkin A;Penkner A;Jantsch V;Gruenbaum Y
通讯作者：
Gruenbaum Y
影响因子：
3.1
作者：
Miki, F;Kurabayashi, A;Niwa, O
通讯作者：
Niwa, O
DOI：
10.1073/pnas.0808602106
发表时间：
2009-02-17
影响因子：
11.1
作者：
Roux, Kyle J.;Crisp, Melissa L.;Burke, Brian
通讯作者：
Burke, Brian
影响因子：
3.3
作者：
Minn, I. L.;Rolls, Melissa M.;Malone, Christian J.
通讯作者：
Malone, Christian J.
影响因子：
10.5
作者：
Tanno, Yuji;Kitajima, Tomoya S.;Watanabe, Yoshinori
通讯作者：
Watanabe, Yoshinori