Mass spectrometry analytical responses to the SARS-CoV2 coronavirus in review.
Mass spectrometry analytical responses to the SARS-CoV2 coronavirus in review.
复制标题
质谱分析响应SARS-CoV 2冠状病毒的审查。
DOI：
10.1016/j.trac.2021.116328
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Downard KM
中科院分区：
文献类型：
--
作者：
Griffin JH;Downard KM
本文回顾了在持续的全球医疗危机中对SARS-CoV2冠状病毒的许多不同的质谱反应。虽然RT-PCR是最普遍的基于分子的监测方法，但质谱检测灵敏度的提高，加上分析的快速性质、测量的高分子精度、高样品吞吐量的机会以及现场测试的潜力，为描述病毒特征和研究病毒感染宿主细胞的分子途径提供了优势。MALDI-TOF质谱法检测生物标志物，利用蛋白质分型策略研究病毒肽，靶向LC-MS分析鉴定临床标本中丰富的肽，病毒蛋白糖型分析，蛋白质组学方法了解感染对宿主细胞的影响，以及即时呼吸分析的检查都进行了探索。本综述参照疫情爆发以来文献中出现的许多研究，组织并说明了这些应用。在这方面，那些质谱法发挥主要作用的研究是重点，只有那些经过同行评审的研究才被引用。
This article reviews the many and varied mass spectrometry based responses to the SARS-CoV2 coronavirus amidst a continuing global healthcare crisis. Although RT-PCR is the most prevalent molecular based surveillance approach, improvements in the detection sensitivities with mass spectrometry coupled to the rapid nature of analysis, the high molecular precision of measurements, opportunities for high sample throughput, and the potential for in-field testing, offer advantages for characterising the virus and studying the molecular pathways by which it infects host cells. The detection of biomarkers by MALDI-TOF mass spectrometry, studies of viral peptides using proteotyping strategies, targeted LC-MS analyses to identify abundant peptides in clinical specimens, the analysis of viral protein glycoforms, proteomics approaches to understand impacts of infection on host cells, and examinations of point-of-care breath analysis have all been explored. This review organises and illustrates these applications with reference to the many studies that have appeared in the literature since the outbreak. In this respect, those studies in which mass spectrometry has a major role are the focus, and only those which have peer-reviewed have been cited.
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