Hydration of proteins: excess partial volumes of water and proteins.
Hydration of proteins: excess partial volumes of water and proteins.
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蛋白质的水合作用：过量的部分体积的水和蛋白质。
DOI：
10.1021/jp300726p
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Aigul V. Khadiullina
中科院分区：
文献类型：
--
作者：
V. Sirotkin;I. Komissarov;Aigul V. Khadiullina
采用高精度光密度法研究蛋白质的水化过程。通过同时监测水和蛋白质在整个水含量范围内的过量部分体积来分析水合过程。使用5种不相关的蛋白质（溶菌酶、胰凝乳蛋白酶原A、卵清蛋白、人血清白蛋白和β-乳球蛋白）作为模型。将所得数据与水和蛋白质的过量偏导数进行比较。结果表明，过量部分量对水和蛋白质状态的变化非常敏感。在最低的水重量分数（w（1））下，过量函数的变化可以主要归因于水的添加。蛋白质从玻璃态到柔性态的转变伴随着过量的部分量的水和蛋白质的显著变化。这种转变出现在水的重量分数为0.06时，带电荷的蛋白质基团被覆盖。当极性和弱相互作用的表面元素都被完全覆盖时，过量的部分量在w（1）> 0.5时达到它们的完全水合值。在最高含水量下，加水对过量量没有显著影响。当w（1）> 0.5时，过量功能的变化可以仅仅归因于蛋白质状态的变化。
High precision densitometry was applied to study the hydration of proteins. The hydration process was analyzed by the simultaneous monitoring of the excess partial volumes of water and the proteins in the entire range of water content. Five unrelated proteins (lysozyme, chymotrypsinogen A, ovalbumin, human serum albumin, and β-lactoglobulin) were used as models. The obtained data were compared with the excess partial enthalpies of water and the proteins. It was shown that the excess partial quantities are very sensitive to the changes in the state of water and proteins. At the lowest water weight fractions (w(1)), the changes of the excess functions can mainly be attributed to water addition. A transition from the glassy to the flexible state of the proteins is accompanied by significant changes in the excess partial quantities of water and the proteins. This transition appears at a water weight fraction of 0.06 when charged groups of proteins are covered. Excess partial quantities reach their fully hydrated values at w(1) > 0.5 when coverage of both polar and weakly interacting surface elements is complete. At the highest water contents, water addition has no significant effect on the excess quantities. At w(1) > 0.5, changes in the excess functions can solely be attributed to changes in the state of the proteins.
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DOI：
10.1016/0167-4838(95)00156-o
发表时间：
1995-11-15
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
COSTANTINO, HR;GRIEBENOW, K;KLIBANOV, A
通讯作者：
KLIBANOV, A
关于伴随生物聚合物构象转变的体积变化。
影响因子：
2.9
作者：
Chalikian,TV;Bresiauer,KJ
通讯作者：
Bresiauer,KJ
DOI：
10.1016/s0378-4347(01)00108-6
发表时间：
2001-05-25
期刊：
JOURNAL OF CHROMATOGRAPHY B
影响因子：
--
作者：
Huntington, JA;Stein, PE
通讯作者：
Stein, PE
DOI：
10.1073/pnas.92.24.10969
发表时间：
1995-11-21
影响因子：
11.1
作者：
GRIEBENOW, K;KLIBANOV, AM
通讯作者：
KLIBANOV, AM