Specification of Cerebellar Progenitors After Heterotopic–Heterochronic Transplantation to the Embryonic CNSIn Vivo and In Vitro
Specification of Cerebellar Progenitors After Heterotopic–Heterochronic Transplantation to the Embryonic CNSIn Vivo and In Vitro
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体内外异位异时移植至胚胎中枢神经系统后小脑祖细胞的规格
DOI：
10.1523/jneurosci.22-16-07132.2002
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
F. Rossi
中科院分区：
文献类型：
--
作者：
B. Carletti;Piercesare Grimaldi;L. Magrassi;F. Rossi
不同的小脑表型是根据胚胎和出生后发育期间的精确时间表产生的。为了评估小脑祖细胞的分化潜力是否在空间和时间上逐渐受到限制，我们检查了胚胎第12天（E12）或出生后第4天（P4）小脑细胞在异位异时移植到子宫内胚胎大鼠大脑或体外器官型中枢神经系统外植体中后的命运。从在β-肌动蛋白启动子的控制下过度表达增强型绿色荧光蛋白的转基因小鼠中分离出的供体细胞，被移植到整个宿主脑干和间脑，而它们很少融入特定的端脑结构。在任何受体部位，绝大多数移植细胞都可以被识别为小脑表型，并且我们没有获得明确的证据表明异位定位的细胞采用了宿主特异性身份。然而，两个捐赠群体表现出不同的发展潜力。 P4 祖细胞专门生成颗粒细胞和分子层中间神经元，表明它们致力于晚期生成的小脑身份，并且对异位异时环境线索不敏感。相比之下，E12前体细胞有潜力产生所有主要的小脑神经元，但这些细胞产生的成体表型在不同的宿主区域是不同的，这表明它们需要指导性的环境信息来获得成熟的身份。因此，小脑前体细胞能够整合到不同的外来大脑区域，在那里它们形成成熟的表型，在移植后存活很长时间，但它们在 E12 时就注定了小脑的命运。胚胎祖细胞最初有能力（尽管可能没有能力）产生所有小脑特性，但它们的潜力逐渐仅限于晚期产生的表型。
The different cerebellar phenotypes are generated according to a precise time schedule during embryonic and postnatal development. To assess whether the differentiative potential of cerebellar progenitors is progressively restricted in space and time we examined the fate of embryonic day 12 (E12) or postnatal day 4 (P4) cerebellar cells after heterotopic–heterochronic transplantation into the embryonic rat brain in utero or into organotypic CNS explants in vitro. Donor cells, isolated from transgenic mice overexpressing the enhanced-green fluorescent protein under the control of the β-actin-promoter, engrafted throughout the host brainstem and diencephalon, whereas they rarely incorporated into specific telencephalic structures. In any recipient site, the vast majority of transplanted cells could be recognized as cerebellar phenotypes, and we did not obtain clear evidence that ectopically located cells adopted host-specific identities. Nevertheless, the two donor populations displayed different developmental potentialities. P4 progenitors exclusively generated granule cells and molecular layer interneurons, indicating that they are committed to late-generated cerebellar identities and not responsive to heterotopic–heterochronic environmental cues. In contrast, E12 precursors had the potential to produce all major cerebellar neurons, but the repertoire of adult phenotypes generated by these cells was different in distinct host regions, suggesting that they require instructive environmental information to acquire mature identities. Thus, cerebellar precursors are able to integrate into different foreign brain regions, where they develop mature phenotypes that survive long after transplantation, but they are committed to cerebellar fates at E12. Embryonic progenitors are initially capable, although likely not competent, to generate all cerebellar identities, but their potential is gradually restricted toward late-generated phenotypes.
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胚胎移植后聚集体中发现的基底神经节前体在异位位置采用纹状体表型。
DOI：
10.1242/dev.125.15.2847
发表时间：
1998
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Magrassi,L;Ehrlich,ME;Butti,G;Pezzotta,S;Govoni,S;Cattaneo,E
通讯作者：
Cattaneo,E
DOI：
10.1073/pnas.94.26.14832
发表时间：
1997-12
影响因子：
11.1
作者：
Daniel A. Lim;Gord Fishell;A. Alvarez-Buylla
通讯作者：
Daniel A. Lim;Gord Fishell;A. Alvarez-Buylla
影响因子：
4.6
作者：
Aparna Desai;S. Mcconnell
通讯作者：
Aparna Desai;S. Mcconnell
纹状体前体采用皮质身份来响应局部线索。
DOI：
10.1242/dev.121.3.803
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Fishell,G
通讯作者：
Fishell,G
嗅觉神经前体细胞的谱系规范取决于连续的细胞相互作用。
DOI：
10.1016/0165-3806(96)00068-5
发表时间：
1996
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Magrassi,L;Graziadei,PP
通讯作者：
Graziadei,PP