Design strategies of fluorescent biosensors based on biological macromolecular receptors.
Design strategies of fluorescent biosensors based on biological macromolecular receptors.
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DOI：
10.3390/s100201355
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Morii T
中科院分区：
文献类型：
--
作者：
Tainaka K;Sakaguchi R;Hayashi H;Nakano S;Liew FF;Morii T
在分子细胞生物学领域，越来越需要荧光生物传感器来检测活细胞中生物学重要分子的真实事件。荧光生物传感器的最新进展不仅对阐明单个生物分子的作用，而且对阐明这些分子之间的动态细胞内关系做出了杰出的贡献。然而，合理的荧光生物传感器的设计策略并不像他们看起来那么成熟。一个贪得无厌的要求，建立一个更普遍的和通用的策略继续提供一个有吸引力的替代方案，所谓的模块化策略，它允许通过一个简单的组合的受体和信号转导器的生物传感器的定制特性的简易制备。本文综述了荧光生物传感器的设计策略的进展，如自发荧光的蛋白质为基础的生物传感器，共价修饰的蛋白质为基础的生物传感器与合成的荧光团，和信号适配体，并突出了洞察到一个给定的受体是如何转换成荧光生物传感器。此外，我们将展示传感器设计的模块化策略的意义。
Fluorescent biosensors to detect the bona fide events of biologically important molecules in living cells are increasingly demanded in the field of molecular cell biology. Recent advances in the development of fluorescent biosensors have made an outstanding contribution to elucidating not only the roles of individual biomolecules, but also the dynamic intracellular relationships between these molecules. However, rational design strategies of fluorescent biosensors are not as mature as they look. An insatiable request for the establishment of a more universal and versatile strategy continues to provide an attractive alternative, so-called modular strategy, which permits facile preparation of biosensors with tailored characteristics by a simple combination of a receptor and a signal transducer. This review describes an overview of the progress in design strategies of fluorescent biosensors, such as auto-fluorescent protein-based biosensors, protein-based biosensors covalently modified with synthetic fluorophores, and signaling aptamers, and highlights the insight into how a given receptor is converted to a fluorescent biosensor. Furthermore, we will demonstrate a significance of the modular strategy for the sensor design.
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影响因子：
2.2
作者：
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通讯作者：
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作者：
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DOI：
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发表时间：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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