Direct Measurement of Cerebrospinal Fluid Production in Mice.
Direct Measurement of Cerebrospinal Fluid Production in Mice.
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DOI：
10.1016/j.celrep.2020.108524
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发表时间：
2020-12-22
期刊：
影响因子：
8.8
通讯作者：
Nedergaard M
中科院分区：
文献类型：
--
作者：
Liu G;Mestre H;Sweeney AM;Sun Q;Weikop P;Du T;Nedergaard M
The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4th ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked. Liu et al. develop a method for direct quantification of cerebrospinal fluid (CSF) production in awake mice. Using this method, the authors evaluate the effect of brain states, ages, sex, anesthetic types, and amyloid-β burden on CSF production.
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影响因子：
3
作者：
Karimy JK;Kahle KT;Kurland DB;Yu E;Gerzanich V;Simard JM
通讯作者：
Simard JM
DOI：
10.1073/pnas.1914017117
发表时间：
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影响因子：
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通讯作者：
Nedergaard M
影响因子：
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通讯作者：
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