Comparative Transcriptome Analyses of Longissimus thoracis Between Pig Breeds Differing in Muscle Characteristics.
Comparative Transcriptome Analyses of Longissimus thoracis Between Pig Breeds Differing in Muscle Characteristics.
复制标题
不同肌肉特征猪品种胸最长肌的比较转录组分析
DOI：
10.3389/fgene.2020.526309
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发表时间：
2020
影响因子：
3.7
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Cai C;Li M;Zhang Y;Meng S;Yang Y;Gao P;Guo X;Cao G;Li B
马深猪(北方中国猪)和大白猪(西部瘦肉猪)从早期发育阶段起，在生长发育、适应性、肌内脂肪含量和能量代谢等方面表现出重要的表型差异。本研究的主要目的是评估这两种基因类型的胸最长肌转录组图谱，以确定导致其分化表型的基因和途径。分别于出生后0d(早期)、90d(中期)和180d(后期)对MS和LW猪的胸最长肌进行取材。在三个时间点共鉴定出3487个差异表达基因(Deg)。样本聚类分析表明，MS的生长速度慢于LW猪。基因表达谱分析表明，多细胞生物生长基因(GHSR、EZR、FOXS1、DRD2、SH3PXD2B、CSF1和TSHR)参与了LW仔猪早期的快速生长。此外，与脂肪酸合成相关的DEGS(ACACA、ACSF3、OXSM、CBR4和HSD17B8)表明，IMF的积累发生在90d左右，直到180d。这些结果为研究MS猪和LW猪胸最长肌的表型差异提供了有价值的资源。
The two breeds, Mashen (MS; a northern China breed) and Large White (LW; a western lean breed) pigs, show important phenotypic differences in growth, adaptability, intramuscular fat (IMF) content, and energy metabolism since early developmental stage. The main aim of this study was the evaluation of longissimus thoracis muscle transcriptome profile of both genetic types to identify genes, pathways responsible for their differentiated phenotype. Longissimus thoracis of MS and LW pigs were sampled at 0 day (early stage), 90 days (middle stage), and 180 days (later stage) after birth. A total of 3,487 differentially expressed genes (DEGs) were identified at the three time points. Sample clustering analysis revealed the slower growth rate of MS than LW pigs. Gene expression pattern analysis revealed that multicellular organism growth genes (GHSR, EZR, FOXS1, DRD2, SH3PXD2B, CSF1, and TSHR) were involved in the fast growth rate of LW pigs at early stage. Furthermore, DEGs (ACACA, ACSF3, OXSM, CBR4, and HSD17B8) functionally related to fatty acid synthesis revealed that IMF accumulation occurred around 90 and up to 180 days. These DEGs provided valuable resource to study the phenotypic difference in longissimus thoracis muscle between MS and LW pigs.
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影响因子：
5.4
作者：
Freeman TC;Ivens A;Baillie JK;Beraldi D;Barnett MW;Dorward D;Downing A;Fairbairn L;Kapetanovic R;Raza S;Tomoiu A;Alberio R;Wu C;Su AI;Summers KM;Tuggle CK;Archibald AL;Hume DA
通讯作者：
Hume DA
中国二花猪和西部杜洛克×（长白×约克夏）商业猪群肉品质性状的全基因组关联分析
DOI：
10.1186/s12711-015-0120-x
发表时间：
2015-05-12
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Liu X;Xiong X;Yang J;Zhou L;Yang B;Ai H;Ma H;Xie X;Huang Y;Fang S;Xiao S;Ren J;Ma J;Huang L
通讯作者：
Huang L
影响因子：
5.6
作者：
Liu Y;Yang X;Jing X;He X;Wang L;Liu Y;Liu D
通讯作者：
Liu D
影响因子：
3.7
作者：
Munoz, Maria;Maria Garcia-Casco, Juan;Silio, Luis
通讯作者：
Silio, Luis
影响因子：
4.4
作者：
Cánovas A;Quintanilla R;Amills M;Pena RN
通讯作者：
Pena RN