Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits.
Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits.
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DOI：
10.1186/1471-2164-11-372
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发表时间：
2010-06-11
期刊：
影响因子：
4.4
通讯作者：
Pena RN
中科院分区：
文献类型：
--
作者：
Cánovas A;Quintanilla R;Amills M;Pena RN
选择提高肌内脂肪含量的品种，将明显改善猪肉的适口性和多汁性，以及腌制产品的感官和感官特性。然而，在人类和模式生物中获得的证据表明，高水平的肌内脂肪可能会改变肌肉的脂质和碳水化合物代谢。我们分析了这个问题，通过确定杜洛克猪的不同表型的13个肥度性状的转录谱。杜洛克猪具有高水平肌内脂肪的强烈倾向，使其成为分析调节肌肉脂质代谢的机制的有价值的模型，这一问题在阐明人类代谢疾病如肥胖和胰岛素抵抗的遗传基础方面具有明显的意义。本文分析了68头杜洛克猪肌肉基因表达谱，这些猪属于两组（高和低），具有脂肪沉积和组成性状的极端表型。微阵列和定量PCR分析表明，与脂肪酸摄取，脂肪生成和三酰甘油合成相关的基因在HIGH猪的肌肉组织中上调，HIGH猪比LOW猪更胖，肌内脂肪含量更高。有趣的是，在HIGH组中脂解基因也显示出mRNA水平增加，表明存在三酰甘油连续合成和降解的循环。与胰岛素信号通路相关的几个基因，通常在肥胖人群中受损，也被上调。最后，与抗原加工和呈递相关的基因在HIGH组中下调。我们的数据表明，增加猪肌内脂肪含量的选择会导致肌肉细胞代谢稳态的转变，但不会破坏。未来的研究翻译后的变化，影响蛋白质的活性或表达，以及有关蛋白质在细胞内的位置的信息，将需要阐明脂质沉积对猪肌肉代谢的影响。
Selection for increasing intramuscular fat content would definitively improve the palatability and juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences obtained in human and model organisms suggest that high levels of intramuscular fat might alter muscle lipid and carbohydrate metabolism. We have analysed this issue by determining the transcriptomic profiles of Duroc pigs with divergent phenotypes for 13 fatness traits. The strong aptitude of Duroc pigs to have high levels of intramuscular fat makes them a valuable model to analyse the mechanisms that regulate muscle lipid metabolism, an issue with evident implications in the elucidation of the genetic basis of human metabolic diseases such as obesity and insulin resistance. Muscle gene expression profiles of 68 Duroc pigs belonging to two groups (HIGH and LOW) with extreme phenotypes for lipid deposition and composition traits have been analysed. Microarray and quantitative PCR analysis showed that genes related to fatty acid uptake, lipogenesis and triacylglycerol synthesis were upregulated in the muscle tissue of HIGH pigs, which are fatter and have higher amounts of intramuscular fat than their LOW counterparts. Paradoxically, lipolytic genes also showed increased mRNA levels in the HIGH group suggesting the existence of a cycle where triacylglycerols are continuously synthesized and degraded. Several genes related to the insulin-signalling pathway, that is usually impaired in obese humans, were also upregulated. Finally, genes related to antigen-processing and presentation were downregulated in the HIGH group. Our data suggest that selection for increasing intramuscular fat content in pigs would lead to a shift but not a disruption of the metabolic homeostasis of muscle cells. Future studies on the post-translational changes affecting protein activity or expression as well as information about protein location within the cell would be needed to to elucidate the effects of lipid deposition on muscle metabolism in pigs.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
46.9
作者：
Canales, Roger D.;Luo, Yuling;Goodsaid, Federico M.
通讯作者：
Goodsaid, Federico M.
DOI：
10.1016/j.beem.2005.04.001
发表时间：
2005-09-01
影响因子：
7.4
作者：
Blaak, EE
通讯作者：
Blaak, EE
影响因子：
3.4
作者：
Bonen, Arend
通讯作者：
Bonen, Arend
DOI：
10.1161/01.atv.14.4.542
发表时间：
1994-04-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
JONES, PJH;LICHTENSTEIN, AH;NAMCHUK, GL
通讯作者：
NAMCHUK, GL