Unraveling incompatibility between wheat and the fungal pathogen Zymoseptoria tritici through apoplastic proteomics.
Unraveling incompatibility between wheat and the fungal pathogen Zymoseptoria tritici through apoplastic proteomics.
复制标题
DOI：
10.1186/s12864-015-1549-6
复制
发表时间：
2015-05-08
期刊：
影响因子：
4.4
通讯作者：
Palmisano G
中科院分区：
文献类型：
--
作者：
Yang F;Li W;Derbyshire M;Larsen MR;Rudd JJ;Palmisano G
半生物营养真菌致病菌小麦酵母菌（Zymoseptoria tritici）引起严重的小麦叶面病害。然而，目前对小麦瘟病菌抗性的分子机制和毒力因子的了解还很不完整。本文研究了寄主小麦和小黑麦在亲和不亲和互作过程中叶片胞外液的蛋白质组学。蛋白质组学分析显示，宿主对生物营养生长的快速反应，包括碳水化合物代谢增强、外胞体防御和应激以及细胞壁强化，可能有助于抵抗。寄主与病原菌的相容性与植物失活的胞外反应以及真菌对氧化胁迫和植物细胞壁扰动的防御有关，随后在坏死性阶段强烈诱导植物防御。为了深入研究抗氧化应激在小麦偃麦草致病性中的作用，我们删除了一个调控抗氧化表达的YAP1转录因子，该转录因子对小麦偃麦草的抗氧化应激有贡献，但不是致病性所必需的。这一结果表明抗氧化剂在真菌中的功能冗余。这些数据表明，不亲和性可能是由于宿主胞外防御的蛋白质组水平激活以及真菌在相互作用的生物营养阶段无法适应胁迫和干扰宿主细胞造成的。本文的在线版本（doi:10.1186/s12864-015-1549-6）包含补充材料，可供授权用户使用。
Hemibiotrophic fungal pathogen Zymoseptoria tritici causes severe foliar disease in wheat. However, current knowledge of molecular mechanisms involved in plant resistance to Z. tritici and Z. tritici virulence factors is far from being complete. The present work investigated the proteome of leaf apoplastic fluid with emphasis on both host wheat and Z. tritici during the compatible and incompatible interactions. The proteomics analysis revealed rapid host responses to the biotrophic growth, including enhanced carbohydrate metabolism, apoplastic defenses and stress, and cell wall reinforcement, might contribute to resistance. Compatibility between the host and the pathogen was associated with inactivated plant apoplastic responses as well as fungal defenses to oxidative stress and perturbation of plant cell wall during the initial biotrophic stage, followed by the strong induction of plant defenses during the necrotrophic stage. To study the role of anti-oxidative stress in Z. tritici pathogenicity in depth, a YAP1 transcription factor regulating antioxidant expression was deleted and showed the contribution to anti-oxidative stress in Z. tritici, but was not required for pathogenicity. This result suggests the functional redundancy of antioxidants in the fungus. The data demonstrate that incompatibility is probably resulted from the proteome-level activation of host apoplastic defenses as well as fungal incapability to adapt to stress and interfere with host cell at the biotrophic stage of the interaction. The online version of this article (doi:10.1186/s12864-015-1549-6) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.7
作者：
Floerl S;Majcherczyk A;Possienke M;Feussner K;Tappe H;Gatz C;Feussner I;Kües U;Polle A
通讯作者：
Polle A
影响因子：
5.3
作者：
Floerl, Saskia;Druebert, Christine;Polle, Andrea
通讯作者：
Polle, Andrea
影响因子：
7.4
作者：
Rudd, Jason J.;Kanyuka, Kostya;Courbot, Mikael
通讯作者：
Courbot, Mikael
影响因子：
4.8
作者：
Koellner, Tobias G.;Schnee, Christiane;Degenhardt, Joerg
通讯作者：
Degenhardt, Joerg
影响因子：
9.4
作者：
Mattsson, M;Schjoerring, JK
通讯作者：
Schjoerring, JK