Verticillium longisporum infection affects the leaf apoplastic proteome, metabolome, and cell wall properties in Arabidopsis thaliana.
Verticillium longisporum infection affects the leaf apoplastic proteome, metabolome, and cell wall properties in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0031435
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Polle A
中科院分区：
文献类型：
--
作者：
Floerl S;Majcherczyk A;Possienke M;Feussner K;Tappe H;Gatz C;Feussner I;Kües U;Polle A
长孢黄萎病（VL）是十字花科重要油料作物中最具破坏性的病害之一。这种真菌在其生命周期的大部分时间里都存在于血管系统的细胞外液中，而传统的杀菌剂无法对其进行控制。为了深入了解质外体中VL-植物相互作用的生物学，在模型十字花科拟南芥中研究了由细胞外蛋白质组和代谢组组成的分泌组以及细胞壁特性。 VL 感染导致细胞壁材料的产生增加，碳水化合物聚合物的组成发生改变并增加木质化。 VL感染植物的质外体中数百种可溶性代谢物的丰度发生了变化，包括信号和防御化合物，例如水杨酸、木酚素和二羟基苯甲酸以及氧脂素的苷。健康叶子的细胞外蛋白质组富含抗真菌蛋白质。 VL 引起六种质外体蛋白（三种过氧化物酶 PRX52、PRX34、P37、丝氨酸羧肽酶 SCPL20、α-半乳糖苷酶 AGAL2 和种芽蛋白样蛋白 GLP3）的特异性增加，这些蛋白具有防御和细胞壁修饰的功能。凝集素样几丁质诱导蛋白（CILLP）的丰度减少。由于大多数诱导蛋白的转录水平直到感染后期（> 20 dpi）才升高，而 CILLP 和 GLP3 的转录水平在较早时间点降低，因此我们的结果可能表明 VL 通过快速下调和延迟植物防御基因的诱导来增强其毒力。
Verticillium longisporum (VL) is one of the most devastating diseases in important oil crops from the family of Brassicaceae. The fungus resides for much time of its life cycle in the extracellular fluid of the vascular system, where it cannot be controlled by conventional fungicides. To obtain insights into the biology of VL-plant interaction in the apoplast, the secretome consisting of the extracellular proteome and metabolome as well as cell wall properties were studied in the model Brassicaceae, Arabidopsis thaliana. VL infection resulted in increased production of cell wall material with an altered composition of carbohydrate polymers and increased lignification. The abundance of several hundred soluble metabolites changed in the apoplast of VL-infected plants including signalling and defence compounds such as glycosides of salicylic acid, lignans and dihydroxybenzoic acid as well as oxylipins. The extracellular proteome of healthy leaves was enriched in antifungal proteins. VL caused specific increases in six apoplast proteins (three peroxidases PRX52, PRX34, P37, serine carboxypeptidase SCPL20, α-galactosidase AGAL2 and a germin-like protein GLP3), which have functions in defence and cell wall modification. The abundance of a lectin-like, chitin-inducible protein (CILLP) was reduced. Since the transcript levels of most of the induced proteins were not elevated until late infection time points (>20 dpi), whereas those of CILLP and GLP3 were reduced at earlier time points, our results may suggest that VL enhances its virulence by rapid down-regulation and delay of induction of plant defence genes.
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影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
5.3
作者：
Floerl, Saskia;Druebert, Christine;Polle, Andrea
通讯作者：
Polle, Andrea
影响因子：
4.8
作者：
Bartsch, Michael;Bednarek, Pawel;Parker, Jane E.
通讯作者：
Parker, Jane E.
影响因子：
4.1
作者：
Cowley, T;Walters, D
通讯作者：
Walters, D
影响因子：
4.4
作者：
Alvarez, S;Goodger, JQD;Schachtman, DP
通讯作者：
Schachtman, DP