Human immunodeficiency virus type 2 envelope glycoprotein: differential CD4 interactions of soluble gp120 versus the assembled envelope complex.
Human immunodeficiency virus type 2 envelope glycoprotein: differential CD4 interactions of soluble gp120 versus the assembled envelope complex.
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人类免疫缺陷病毒 2 型包膜糖蛋白：可溶性 gp120 与组装包膜复合物的差异 CD4 相互作用。
DOI：
10.1016/0042-6822(92)90311-c
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
Compans,RW
中科院分区：
文献类型：
--
作者：
Mulligan,MJ;RitterJr,GD;Chaikin,MA;Yamshchikov,GV;Kumar,P;Hahn,BH;Sweet,RW;Compans,RW
Utilizing a recombinant vaccinia expression system, we investigated the biological properties and CD4 receptor interactions of the envelope glycoproteins of a noncytopathic human immunodeficiency virus type 2 strain, termed HIV-2/ST, and a highly cytopathic variant derived from it. The efficiency and host cell range of syncytium formation by the recombinant glycoproteins of both viruses were highly restricted compared to those of prototypic strains of HIV (HIV-2/ROD or HIV-1/11113). However, the glycoprotein of cytopathic but not wild-type ST generated numerous large syncytia in the human T-cell line Sup T1 from which it was derived. A single cell line (Molt 4 clone 8) was permissive to fusion by both wild-type and cytopathic ST envelopes, but only the glycoprotein of cytopathic ST could be inhibited with a soluble form of the viral receptor CD4 (sCD4). While these results indicated major differences in the envelope glycoprotein-CD4 receptor interactions of wild-type versus cytopathic ST, direct and competition binding assays utilizing soluble external glycoprotein (SU) and sCD4 surprisingly revealed equivalent low binding affinity for both viruses. From these experiments we conclude that relevant biological properties (e.g., CD4 binding, cytopathic potential, and sCD4 neutralization) of HIV viruses which differ in their pathogenic potential are reflected in the sCD4 interactions of the assembled native envelope complex (as on cell or virion surfaces) but not the soluble SU glycoprotein.
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传染性非细胞病变性 HIV-2 的 env 蛋白在合胞体形成方面存在缺陷。
影响因子：
1.5
作者：
Mulligan,MJ;Kumar,P;Hui,HX;Owens,RJ;RitterJr,GD;Hahn,BH;Compans,RW
通讯作者：
Compans,RW
影响因子：
64.5
作者：
ARTHOS, J;DEEN, KC;SWEET, RW
通讯作者：
SWEET, RW
重组 HIV-1 和 HIV-2 SU 糖蛋白与 sCD4 的结合。
DOI：
--
发表时间：
1991
期刊：
Journal of Acquired Immune Deficiency Syndromes
影响因子：
--
作者：
J. Moore;Y. Morikawa;I. Jones
通讯作者：
I. Jones
HIV-1 和 HIV-2 与 CD4 相互作用的差异。
DOI：
--
发表时间：
1990
期刊：
Journal of Acquired Immune Deficiency Syndromes
影响因子：
--
作者：
David J. Looney;S. Hayashi;M. Nicklas;Redfield Rr;S. Broder;F. Wong;H. Mitsuya
通讯作者：
H. Mitsuya
DOI：
10.1073/pnas.85.24.9580
发表时间：
1988-12
影响因子：
11.1
作者：
R. Willey;J. Bonifacino;B. Potts;Malcolma . Martin;R. Klausner
通讯作者：
R. Willey;J. Bonifacino;B. Potts;Malcolma . Martin;R. Klausner