miR-324-3p suppresses migration and invasion by targeting WNT2B in nasopharyngeal carcinoma.
miR-324-3p suppresses migration and invasion by targeting WNT2B in nasopharyngeal carcinoma.
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miR-324-3p 通过靶向 WNT2B 抑制鼻咽癌的迁移和侵袭。
DOI：
10.1186/s12935-016-0372-8
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发表时间：
2017
影响因子：
5.8
通讯作者：
Qiu Y
中科院分区：
文献类型：
--
作者：
Liu C;Li G;Yang N;Su Z;Zhang S;Deng T;Ren S;Lu S;Tian Y;Liu Y;Qiu Y
鼻咽癌是头颈部上皮性恶性肿瘤，具有较强的侵袭和转移能力。我们前期的研究表明，miR-324- 3 p作为肿瘤抑制因子，可以通过靶向WNT 2B调节鼻咽癌细胞的放射抗性。本研究旨在探讨miR-324- 3 p在鼻咽癌细胞迁移和侵袭中的作用。采用真实的实时定量PCR检测细胞和组织中miR-324- 3 p和WNT 2B mRNA的表达水平，采用Western blotting检测WNT 2B蛋白的表达水平。采用创伤愈合实验和transwell侵袭实验检测细胞的迁移和侵袭能力。miR-324- 3 p的异位表达或其靶基因WNT 2B的沉默可显著抑制鼻咽癌细胞的迁移和侵袭能力。同时，miR-324- 3 p在鼻咽癌细胞中的表达变化可影响上皮间质转化（EMT）生物标志物E-cadherin和Vimentin的表达水平。鼻咽癌组织中miR-324- 3 p表达明显下调，WNT 2B表达明显上调。miR-324- 3 p和WNT 2B的表达水平与鼻咽癌的T分期、临床分期和颈淋巴结转移密切相关（P <0. 05）。miR-324- 3 p可通过靶向WNT 2B抑制鼻咽癌的迁移和侵袭，miR-324- 3 p/WNT 2B通路可能为鼻咽癌的治疗提供新的线索。本文的在线版本（doi：10.1186/s12935-016-0372-8）包含补充材料，可供授权用户使用。
Nasopharyngeal carcinoma (NPC) is a malignant epithelial carcinoma of the head and neck with strong ability of invasion and metastasis. Our previous study indicated that miR-324-3p, as a tumor-suppressive factor, could regulate radioresistance of NPC cells by targeting WNT2B. The purpose of this study is to investigate the role of miR-324-3p on migration and invasion in NPC cells. Quantitative real time PCR was applied to measure the expression level of miR-324-3p and WNT2B mRNA in both cells and tissues, and the expression level of WNT2B protein was determined by western blotting. The capacity of migration and invasion were tested by using wound healing and transwell invasion assay. Ectopic expression of miR-324-3p or silencing its target gene WNT2B could dramatically suppress migration and invasion capacity of NPC cells. Meanwhile, the alterations of miR-324-3p in NPC cells could influence the expression level of the biomarkers of epithelial-mesenchymal transition (EMT), including E-cadherin and Vimentin. Moreover, the expression of miR-324-3p was obviously downregulated and WNT2B was significantly upregulated in NPC tissues. The expression levels of miR-324-3p and WNT2B were closely correlated with T stage, clinic stage and cervical lymph node metastasis of NPC (P < 0.05). miR-324-3p could suppress the migration and invasion of NPC by targeting WNT2B and the miR-324-3p/WNT2B pathway possibly provide new potential therapeutic clues for NPC. The online version of this article (doi:10.1186/s12935-016-0372-8) contains supplementary material, which is available to authorized users.
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