Temporal and spatial patterning of transgene expression by near-infrared irradiation.
Temporal and spatial patterning of transgene expression by near-infrared irradiation.
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DOI：
10.1016/j.biomaterials.2014.06.009
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发表时间：
2014-09
期刊：
影响因子：
14
通讯作者：
Vilaboa, Nuria
中科院分区：
文献类型：
--
作者：
Martin-Saavedra, Francisco M.;Cebrian, Virginia;Gomez, Leyre;Lopez, Daniel;Arruebo, Manuel;Wilson, Christopher G.;Franceschi, Renny T.;Voellmy, Richard;Santamaria, Jesus;Vilaboa, Nuria
我们研究了近红外（NIR）光是否可以用于等离子体细胞构建中的转基因表达模式。利用等离子体表面吸收峰在750 nm处的中空金纳米粒子作为纤维蛋白基水凝胶的填料，该波长在所谓的“组织光学窗口”内。这些复合材料有效地将近红外光子能量转化为热量，并装载了含有热激活和配体依赖性基因开关的转基因细胞，用于调节转基因表达。在配体存在的情况下，近红外激光照射触发的转基因表达的三维模式忠实地匹配等离子体细胞结构的照射区域。这种非侵入性技术被证明可用于远程控制转基因血管内皮生长因子在体内的时空生物利用度。近红外辐射的空间控制与安全、定时的转基因诱导相结合，在再生医学领域的工程组织中具有很高的应用潜力。
We investigated whether near-infrared (NIR) light could be employed for patterning transgene expression in plasmonic cell constructs. Hollow gold nanoparticles with a plasmon surface band absorption peaking at ~750 nm, a wavelength within the so called “tissue optical window”, were used as fillers in fibrin-based hydrogels. These composites, which efficiently transduce NIR photon energy into heat, were loaded with genetically-modified cells that harbor a heat-activated and ligand-dependent gene switch for regulating transgene expression. NIR laser irradiation in the presence of ligand triggered 3-dimensional patterns of transgene expression faithfully matching the illuminated areas of plasmonic cell constructs. This noninvasive technology was proven useful for remotely controlling in vivo the spatiotemporal bioavailability of transgenic vascular endothelial growth factor. The combination of spatial control by means of NIR irradiation along with safe and timed transgene induction presents a high application potential for engineering tissues in regenerative medicine scenarios.
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DOI：
10.1161/circep.111.967216
发表时间：
2012-04
期刊：
Circulation. Arrhythmia and electrophysiology
影响因子：
--
作者：
Laughner JI;Sulkin MS;Wu Z;Deng CX;Efimov IR
通讯作者：
Efimov IR
影响因子：
14
作者：
Lorentz, Kristen M.;Kontos, Stephan;Hubbell, Jeffrey A.
通讯作者：
Hubbell, Jeffrey A.
影响因子：
2.8
作者：
Park, Keun-Hong;Kim, Hyemin;Na, Kun
通讯作者：
Na, Kun
影响因子：
12.4
作者：
Vilaboa, N;Fenna, M;Voellmy, R
通讯作者：
Voellmy, R
影响因子：
10.4
作者：
Sperzel, M.;Huetter, J.
通讯作者：
Huetter, J.