Regulatory effect of dopamine on spatial properties of horizontal cells in carp retina
Regulatory effect of dopamine on spatial properties of horizontal cells in carp retina
复制标题
多巴胺对鲤鱼视网膜水平细胞空间特性的调节作用
DOI：
10.1523/jneurosci.04-05-01271.1984
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发表时间：
1984
影响因子：
2.5
通讯作者：
S. Kato
中科院分区：
文献类型：
--
作者：
T. Teranishi;Koroku Negishi, 'and;S. Kato
三种类型的光诱导反应（L-，RG-和YRB型S-电位）记录从离体视网膜的鲤鱼（鲤鱼）被确定为他们的光谱响应，后来通过形态定位的记录点标记的细胞内荧光黄（LY）。水平细胞在一个给定的层，产生上述响应类型之一，通过间隙连接电耦合，使注入LY通常扩散到几个相邻的细胞。通过扩大光点的直径（0.25至4.0 mm）并使光点（0.5 mm直径）沿沿着穿过中间记录点的4 mm直线移动，来检查三种类型响应的空间特性。在正常视网膜中，L-、RG-和YRB -型反应的反应幅度随斑点位移的半衰减距离依次较短，相应地，在相同细胞顺序下，染料扩散面积也较窄。多巴胺（DA; 10 - 20 μ M）作用于离体视网膜下的玻璃体液，通过增加对中心点的反应幅度和降低对远处点的反应幅度，改变了所有类型反应的空间特性，并将细胞内LY限制在单个注射细胞内。与此相反，在视网膜DA interplexiform细胞已被剥夺了事先破坏与神经毒素，6-羟基多巴胺，反应的幅度变小，而半衰期的距离是长1.1至1.6倍，这取决于细胞类型，和染料扩散面积在所有类型的细胞变得更广泛的2倍，相比，在正常视网膜。（250字处删节）
Three types of light-induced response (L-, RG-, and YRB -type S- potentials) recorded from isolated retinas of the carp (Cyprinus carpio) were identified by their spectral response and later by morphological localization of the recording sites marked with an intracellular Lucifer Yellow (LY). Horizontal cells in a given layer, generating one of the above response types, are electrically coupled via gap junctions, so that the injected LY normally diffused to several neighboring cells. The spatial property of the three types of responses was examined by enlarging the diameter of a light spot (0.25 to 4.0 mm) and displacing the spot (0.5 mm diameter) along a straight 4-mm line which passed over the recording point at the middle. In normal retinas, the half-decay distance of response amplitude with spot displacement was shorter in the order of L-, RG-, and YRB -type responses, and correspondingly the dye diffusion area was narrower in the same order of cells. Dopamine (DA; 10 to 20 microM), applied to the vitreous fluid beneath the isolated retina, altered the spatial property of all types of responses by increasing the amplitude of responses to central spots and decreasing that of those to distant spots, and it restricted the intracellular LY to single injected cells. In contrast, in retinas from which DA interplexiform cells had been deprived by prior destruction with a neurotoxin, 6-hydroxydopamine, the amplitude of responses became smaller while the half-decay distance was longer by 1.1- to 1.6-fold, depending upon the cell type, and the dye diffusion area in all types of cells became wider by 2-fold as compared to those in normal retinas.(ABSTRACT TRUNCATED AT 250 WORDS)
来自鲤鱼视网膜的分离水平细胞证明了环 AMP 的多巴胺依赖性积累。
DOI：
10.1073/pnas.78.12.7825
发表时间：
1981
影响因子：
11.1
作者：
VanBuskirk,R;Dowling,JE
通讯作者：
Dowling,JE