Predictive value of progression-related gene classifier in primary non-muscle invasive bladder cancer.
Predictive value of progression-related gene classifier in primary non-muscle invasive bladder cancer.
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DOI：
10.1186/1476-4598-9-3
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发表时间：
2010-01-08
期刊：
影响因子：
37.3
通讯作者：
Bae SC
中科院分区：
文献类型：
--
作者：
Kim WJ;Kim EJ;Kim SK;Kim YJ;Ha YS;Jeong P;Kim MJ;Yun SJ;Lee KM;Moon SK;Lee SC;Cha EJ;Bae SC
虽然已经确定了膀胱癌预后的几种分子标志物，但当前预后标志物的价值有限，因此需要新的膀胱癌结果分子指标。本研究的目的是确定与膀胱癌疾病预后相关的遗传特征。我们使用 272 个原发性膀胱癌标本进行微阵列分析和实时逆转录聚合酶链反应 (RT-PCR) 分析。对随机选择的 165 例原发性膀胱癌标本作为原始队列进行了微阵列基因表达分析。应用风险评分对预后相关基因分类进行分层。与预后相关的基因分类器根据肿瘤侵袭性（非肌层浸润性膀胱癌[NMIBC]和肌层浸润性膀胱癌[MIBC]）和预后进行单独分析。我们使用 RT-PCR 在原始 (165) 和独立 (107) 队列中验证了选定的基因分类器。通过微阵列数据分析鉴定出与 NMIBC 患者疾病进展相关的 97 个基因。选择 8 个基因（NMIBC 中的进展相关基因分类器）进行 RT-PCR。 NMIBC 患者的进展相关基因分类与原始队列和独立队列中的进展密切相关。此外，良好预后特征组中没有 NMIBC 患者出现癌症进展。我们确定了与进展相关的基因分类器，对于确定 NMIBC 的疾病结果具有很强的预测价值。该基因分类器可以帮助选择可能受益于更积极的治疗干预或监测的 NMIBC 患者。
While several molecular markers of bladder cancer prognosis have been identified, the limited value of current prognostic markers has created the need for new molecular indicators of bladder cancer outcomes. The aim of this study was to identify genetic signatures associated with disease prognosis in bladder cancer. We used 272 primary bladder cancer specimens for microarray analysis and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis. Microarray gene expression analysis of randomly selected 165 primary bladder cancer specimens as an original cohort was carried out. Risk scores were applied to stratify prognosis-related gene classifiers. Prognosis-related gene classifiers were individually analyzed with tumor invasiveness (non-muscle invasive bladder cancer [NMIBC] and muscle invasive bladder cancer [MIBC]) and prognosis. We validated selected gene classifiers using RT-PCR in the original (165) and independent (107) cohorts. Ninety-seven genes related to disease progression among NMIBC patients were identified by microarray data analysis. Eight genes, a progression-related gene classifier in NMIBC, were selected for RT-PCR. The progression-related gene classifier in patients with NMIBC was closely correlated with progression in both original and independent cohorts. Furthermore, no patient with NMIBC in the good-prognosis signature group experienced cancer progression. We identified progression-related gene classifier that has strong predictive value for determining disease outcome in NMIBC. This gene classifier could assist in selecting NMIBC patients who might benefit from more aggressive therapeutic intervention or surveillance.
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影响因子：
158.5
作者：
Lossos, IS;Czerwinski, DK;Levy, R
通讯作者：
Levy, R
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168.9
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Schellhammer, Paul F.
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1.6
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