New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling.
New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling.
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DOI：
10.1084/jem.20042524
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发表时间：
2005-06-06
期刊：
影响因子：
--
通讯作者：
Staal FJ
中科院分区：
文献类型：
--
作者：
Dik WA;Pike-Overzet K;Weerkamp F;de Ridder D;de Haas EF;Baert MR;van der Spek P;Koster EE;Reinders MJ;van Dongen JJ;Langerak AW;Staal FJ
为了更深入地了解T细胞发育过程中T细胞受体（TCR）基因重排的启动和调控，我们通过定量PCR分析了9个连续T细胞发育阶段的TCR基因重排，包括CD34+ lin -脐带血细胞作为参考。同一阶段使用DNA微阵列进行基因表达谱分析。我们发现TCR基因座以高度有序的方式重排（TCRD-TCRG-TCRB-TCRA），并且起始的Dδ2-Dδ3重排发生在最不成熟的CD34+CD38−CD1a−阶段。TCRB重排始于CD34+CD38+CD1a -阶段，完整的帧内TCRB重排首次在未成熟的单阳性阶段被检测到。TCRB重排数据和PTCRA （pTα）表达模式表明，人类TCRB β选择发生在CD34+CD38+CD1a+阶段。通过将TCR重排数据与基因表达数据相结合，我们确定了TCR重组启动/调控的候选因子。我们的数据表明，一些关键事件发生的时间比之前假设的要早；因此，人类T细胞的发育比以前报道的更类似于小鼠T细胞的发育。
To gain more insight into initiation and regulation of T cell receptor (TCR) gene rearrangement during human T cell development, we analyzed TCR gene rearrangements by quantitative PCR analysis in nine consecutive T cell developmental stages, including CD34+ lin− cord blood cells as a reference. The same stages were used for gene expression profiling using DNA microarrays. We show that TCR loci rearrange in a highly ordered way (TCRD-TCRG-TCRB-TCRA) and that the initiating Dδ2-Dδ3 rearrangement occurs at the most immature CD34+CD38−CD1a− stage. TCRB rearrangement starts at the CD34+CD38+CD1a− stage and complete in-frame TCRB rearrangements were first detected in the immature single positive stage. TCRB rearrangement data together with the PTCRA (pTα) expression pattern show that human TCRβ-selection occurs at the CD34+CD38+CD1a+ stage. By combining the TCR rearrangement data with gene expression data, we identified candidate factors for the initiation/regulation of TCR recombination. Our data demonstrate that a number of key events occur earlier than assumed previously; therefore, human T cell development is much more similar to murine T cell development than reported before.
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DOI：
10.1073/pnas.1932643100
发表时间：
2003-09-30
影响因子：
11.1
作者：
Morshead, KB;Ciccone, DN;Oettinger, MA
通讯作者：
Oettinger, MA
DOI：
10.1073/pnas.95.21.12522
发表时间：
1998-10-13
影响因子：
11.1
作者：
Capone, M;Hockett, RD;Zlotnik, A
通讯作者：
Zlotnik, A
影响因子：
4.4
作者：
Hoffmann, R;Bruno, L;Melchers, F
通讯作者：
Melchers, F
影响因子：
32.4
作者：
Wolfer, A;Wilson, A;Radtke, F
通讯作者：
Radtke, F
影响因子：
32.4
作者：
Villey, I;Caillol, D;deVillartay, JP
通讯作者：
deVillartay, JP