The cyanobacterial circadian system: from biophysics to bioevolution.
The cyanobacterial circadian system: from biophysics to bioevolution.
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DOI：
10.1146/annurev-biophys-042910-155317
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发表时间：
2011
影响因子：
12.4
通讯作者：
Egli M
中科院分区：
文献类型：
--
作者：
Johnson CH;Stewart PL;Egli M
最近的研究揭示了蓝藻生物钟的分子机制，并发现它对包括全球基因表达在内的细胞过程施加普遍控制。事实上，整个染色体每天都经历着拓扑/紧凑的循环！昼夜节律系统包括翻译后振荡器(PTO)和转录/翻译反馈环(TTFL)。三种纯化的蛋白(KaIA、Kaib和KaiC)和三磷酸腺苷(ATP)可在体外重组PTO。这些是唯一可以获得高分辨率结构的昼夜节律蛋白质。这一纳米链中的相与KaiC的关键磷酸化有关。结构上的考虑阐明了KaiABC振荡器单向棘轮的机制。完整的活体系统模型对于我们理解包括哺乳动物在内的高等生物的生物钟有着重要的意义。结构、生物物理和生化方法的结合使我们对生物计时的分子机制的理解达到了前所未有的水平。
Recent studies have unveiled the molecular machinery responsible for the biological clock in cyanobacteria and found that it exerts pervasive control over cellular processes including global gene expression. Indeed, the entire chromosome undergoes daily cycles of topology/compaction! The circadian system comprises both a posttranslational oscillator (PTO) and a transcriptional/translational feedback loop (TTFL). The PTO can be reconstituted in vitro with three purified proteins (KaiA, KaiB, and KaiC) and ATP. These are the only circadian proteins for which high-resolution structures are available. Phase in this nanoclockwork has been associated with key phosphorylations of KaiC. Structural considerations illuminate the mechanism by which the KaiABC oscillator ratchets unidirectionally. Models of the complete in vivo system have important implications for our understanding of circadian clocks in higher organisms, including mammals. The conjunction of structural, biophysical, and biochemical approaches to this system has brought our understanding of the molecular mechanisms of biological timekeeping to an unprecedented level.
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作者：
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通讯作者：
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