Measurement of Japanese cedar pollen-specific IgE in nasal secretions.
Measurement of Japanese cedar pollen-specific IgE in nasal secretions.
复制标题
鼻分泌物中日本柳杉花粉特异性 IgE 的测定。
DOI：
10.2332/allergolint.13-oa-0668
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Takeuchi
中科院分区：
文献类型：
--
作者：
H. Sakaida;S. Masuda;K. Takeuchi
背景
日本雪松花粉(JCP)是日本季节性变应性鼻炎最常见的过敏原。关于患有或不患有日本雪松花粉病的人局部产生免疫球蛋白(Ig)E的情况知之甚少。本研究的目的是测量鼻分泌物中JCP特异性IgE的水平，并确定与血清水平的相关性。
方法
46名受试者包括24名有症状的受试者、9名对JCP敏感的无症状受试者和13名未对JCP致敏的受试者。取日本雪松散布期间的鼻分泌物，用CAP-荧光酶免疫法测定JCP特异性IgE水平。用同样的方法测定血清JCP特异性IgE和总IgE。
结果
在46名受试者中，43名受试者的鼻分泌物中可检测到JCP特异性IgE。与症状发展无关，致敏受试者鼻腔分泌物中JCP特异性IgE水平高于未致敏受试者。在所有43名受试者中，鼻腔分泌物和血清中JCP特异性IgE水平之间存在显著的中等相关性。按受试者分组，只有有症状的受试者在鼻分泌物和血清中的JCP特异性IgE水平之间显示出显著的相关性。
结论
我们的结果暗示，鼻分泌物中JCP特异性的IgE与日本雪松花粉症的致敏作用有一定的关联。因此，鼻腔分泌物中变应原特异性IgE水平可作为变应性鼻炎患者的替代诊断指标。
BACKGROUND
Japanese cedar pollen (JCP) is the most common allergen for seasonal allergic rhinitis in Japan. Little is known about local production of immunoglobulin (Ig)E in people with or without Japanese cedar pollinosis. The aims of this study were to measure levels of JCP-specific IgE in nasal secretions and determine correlations with levels in serum.
METHODS
Forty-six subjects were enrolled in this study, comprising 24 symptomatic subjects, 9 asymptomatic subjects sensitized to JCP, and 13 subjects not sensitized to JCP. Nasal secretions were obtained during a period of Japanese cedar dispersal, and levels of JCP-specific IgE were measured with CAP-fluorescent enzyme immunoassay. Serum JCP-specific IgE and total IgE were also measured using the same method.
RESULTS
Among the 46 subjects enrolled, JCP-specific IgE in nasal secretions was measureable in 43 subjects. Irrespective of symptom development, sensitized subjects showed higher levels of JCP-specific IgE in nasal secretions than non-sensitized subjects. A significant moderate correlation was observed between JCP-specific IgE levels in nasal secretions and serum in all 43 subjects. With stratification by subject group, only symptomatic subjects showed a substantial correlation between JCP-specific IgE levels in nasal secretions and serum.
CONCLUSIONS
Our results imply a certain association between JCP-specific IgE in nasal secretions and sensitization of Japanese cedar pollinosis. Therefore, levels of allergen-specific IgE in nasal secretions can be used as an alternative diagnostic marker for allergic rhinitis patients.
影响因子：
2.8
作者：
Sakashita, Masafumi;Hirota, Tomomitsu;Fujieda, Shigeharu
通讯作者：
Fujieda, Shigeharu