Assessing Onchocerca volvulus Intensity of Infection and Genetic Diversity Using Mitochondrial Genome Sequencing of Single Microfilariae Obtained before and after Ivermectin Treatment.
Assessing Onchocerca volvulus Intensity of Infection and Genetic Diversity Using Mitochondrial Genome Sequencing of Single Microfilariae Obtained before and after Ivermectin Treatment.
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DOI：
10.3390/pathogens12070971
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发表时间：
2023-07-24
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--
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Onchocerciasis is a neglected tropical disease targeted for elimination using ivermectin mass administration. Ivermectin kills the microfilariae and temporarily arrests microfilariae production by the macrofilariae. We genotyped 436 microfilariae from 10 people each in Ituri, Democratic Republic of the Congo (DRC), and Maridi County, South Sudan, collected before and 4–5 months after ivermectin treatment. Population genetic analyses identified 52 and 103 mitochondrial DNA haplotypes among the microfilariae from DRC and South Sudan, respectively, with few haplotypes shared between people. The percentage of genotype-based correct assignment to person within DRC was ~88% and within South Sudan ~64%. Rarefaction and extrapolation analysis showed that the genetic diversity in DRC, and even more so in South Sudan, was captured incompletely. The results indicate that the per-person adult worm burden is likely higher in South Sudan than DRC. Analyses of haplotype data from a subsample (n = 4) did not discriminate genetically between pre- and post-treatment microfilariae, confirming that post-treatment microfilariae are not the result of new infections. With appropriate sampling, mitochondrial haplotype analysis could help monitor changes in the number of macrofilariae in a population as a result of treatment, identify cases of potential treatment failure, and detect new infections as an indicator of continuing transmission.
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影响因子：
3
作者：
Colebunders R;Njamnshi AK;van Oijen M;Mukendi D;Kashama JM;Mandro M;Gumisiriza N;Preux PM;Suykerbuyk P;Idro R
通讯作者：
Idro R
影响因子：
3.8
作者：
通讯作者：
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影响因子：
3.8
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通讯作者：
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影响因子：
2.4
作者：
Bottomley, Christian;Isham, Valerie;Basanez, Maria-Gloria
通讯作者：
Basanez, Maria-Gloria
DOI：
10.1073/pnas.0906176106
发表时间：
2009-09-29
影响因子：
11.1
作者：
Churcher, Thomas S.;Pion, Sebastien D. S.;Basanaz, Maria-Gloria
通讯作者：
Basanaz, Maria-Gloria