PEG3 Interacts with KAP1 through KRAB-A.
PEG3 Interacts with KAP1 through KRAB-A.
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DOI：
10.1371/journal.pone.0167541
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kim J
中科院分区：
文献类型：
--
作者：
He H;Ye A;Kim H;Kim J
Peg 3（Paternally Expressed Gene 3）是一种印迹基因，编码锌指DNA结合蛋白。Peg 3本身位于KRAB-A（Kruppel相关盒）锌指基因簇的中间。其外显子7编码的氨基酸序列也显示与KRAB-A的序列相似性，表明Peg 3为含KRAB的锌指基因。正如预测的那样，PEG 3蛋白与KAP 1共免疫沉淀，KAP 1是一种与KRAB-A相互作用的共阻遏物。一系列后续实验进一步证明，PEG 3的外显子7确实负责其与KAP 1的物理相互作用。ChIP和启动子分析也表明，PEG 3可能通过KAP 1介导的抑制机制控制其下游基因。总的来说，目前的研究确定PEG 3是一种含KRAB的锌指蛋白，与辅助阻遏蛋白KAP 1相互作用。
Peg3 (Paternally Expressed Gene 3) is an imprinted gene that encodes a zinc finger DNA-binding protein. Peg3 itself is localized in the middle of a KRAB-A (Kruppel-Associated Box) zinc finger gene cluster. The amino acid sequence encoded by its exon 7 also shows sequence similarity to that of KRAB-A, suggesting Peg3 as a KRAB-containing zinc finger gene. As predicted, the PEG3 protein was co-immunoprecipitated with KAP1, a co-repressor that interacts with KRAB-A. A series of follow-up experiments further demonstrated that the exon 7 of PEG3 is indeed responsible for its physical interaction with KAP1. ChIP and promoter assays also indicated that PEG3 likely controls its downstream genes through the KAP1-mediated repression mechanism. Overall, the current study identifies PEG3 as a KRAB-containing zinc finger protein that interacts with the co-repressor protein KAP1.
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