Intravital imaging of human prostate cancer using viral nanoparticles targeted to gastrin-releasing Peptide receptors.
Intravital imaging of human prostate cancer using viral nanoparticles targeted to gastrin-releasing Peptide receptors.
复制标题
DOI：
10.1002/smll.201000435
复制
发表时间：
2011-06-20
期刊：
影响因子：
13.3
通讯作者：
Lewis, John D.
中科院分区：
文献类型：
--
作者：
Steinmetz, Nicole F.;Ablack, Amber L.;Hickey, Jennifer L.;Ablack, Jailal;Manocha, Bhavik;Mymryk, Joe S.;Luyt, Leonard G.;Lewis, John D.
多价纳米粒子在溶解性、结合亲和力和摄取性方面具有几个关键优势，使其特别适合于分子成像应用。本文报道了针对人前列腺癌中过度表达的胃泌素释放肽受体的近红外病毒纳米粒的逐步合成和表征。泛蛙素类似物[β-ALA11，Phe13，Nle14]蛙素-(7-14)通过铜(I)催化的叠氮-炔环加成反应与用近红外染料(Alexa Fluor647)和聚乙二醇官能化的豌豆花叶病毒颗粒偶联。体外靶向和摄取人前列腺癌PC-3细胞。使用活体成像技术，在鸡绒毛膜尿囊膜模型上使用人前列腺癌异种移植模型观察到肿瘤归巢。这种病毒纳米颗粒平台的进一步开发可能会为潜在的临床非侵入性分子成像策略打开大门。
Multivalent nanoparticles have several key advantages in terms of solubility, binding avidity, and uptake, making them particularly well suited to molecular imaging applications. Herein is reported the stepwise synthesis and characterization of NIR viral nanoparticles targeted to gastrin-releasing peptide receptors that are over-expressed in human prostate cancers. The pan-bombesin analogue, [β-Ala11, Phe13, Nle14]bombesin-(7–14), is conjugated to cowpea mosaic virus particles functionalized with an NIR dye (Alexa Fluor 647) and polyethylene glycol (PEG) using the copper(I)-catalyzed azide-alkyne cycloaddition reaction. Targeting and uptake in human PC-3 prostate cells is demonstrated in vitro. Tumor homing is observed using human prostate tumor xenografts on the chicken chorioallantoic membrane model using intravital imaging. Further development of this viral nanoparticle platform may open the door to potential clinical noninvasive molecular imaging strategies.
登录
查看更多内容
影响因子：
4.7
作者：
Baidoo, KE;Lin, KS;Wagner, HN
通讯作者：
Wagner, HN
影响因子：
6.7
作者：
Koudelka KJ;Destito G;Plummer EM;Trauger SA;Siuzdak G;Manchester M
通讯作者：
Manchester M
影响因子：
10.8
作者：
Maeda, H;Wu, J;Hori, K
通讯作者：
Hori, K
影响因子：
7.3
作者：
Montet, Xavier;Funovics, Martin;Josephson, Lee
通讯作者：
Josephson, Lee
影响因子：
3.1
作者：
La Bella, R;Garcia-Garayoa, E;Schubiger, PA
通讯作者：
Schubiger, PA