The genome-wide early temporal response of Saccharomyces cerevisiae to oxidative stress induced by cumene hydroperoxide.
The genome-wide early temporal response of Saccharomyces cerevisiae to oxidative stress induced by cumene hydroperoxide.
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DOI：
10.1371/journal.pone.0074939
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shulaev V
中科院分区：
文献类型：
--
作者：
Sha W;Martins AM;Laubenbacher R;Mendes P;Shulaev V
氧化应激是一个众所周知的生物学过程，发生在所有呼吸细胞中，并参与衰老和凋亡等病理生理过程。氧化应激剂包括过氧化物，例如过氧化氢、氢过氧化枯烯和氢过氧化亚油酸、硫醇氧化剂二酰胺和甲萘醌（一种超氧化物产生剂）等。本研究分析了早期的时间全基因组的转录反应的酿酒酵母氧化应激诱导的芳香族过氧化物枯烯氢过氧化物。通过使用时间控制、生物复制和良好控制的生长条件，获得的准确数据集提供了该过程早期动态的详细情况。我们确定了一组基因以前没有牵连的氧化应激反应，包括几个转录调控显示快速的瞬时反应，这表明在转录重编程的协调过程。我们讨论了谷胱甘肽，硫氧还蛋白和活性氧清除系统，蛋白酶体和戊糖磷酸途径的作用。数据驱动的表达模式聚类确定了一个特定的簇，主要由已知受Yap1p和Skn7p转录因子调控的基因组成，强调了它们在氧化剂转录反应中的介导作用。我们的结果与过氧化氢的数据报告的比较确定了664个基因，特异性地响应氢过氧化枯烯，这两个过氧化物不同的转录反应。仅由氢过氧化枯烯上调的基因主要与细胞膜和细胞壁以及蛋白水解过程有关，而仅由这种芳香族过氧化物下调的基因则与线粒体功能有关。
Oxidative stress is a well-known biological process that occurs in all respiring cells and is involved in pathophysiological processes such as aging and apoptosis. Oxidative stress agents include peroxides such as hydrogen peroxide, cumene hydroperoxide, and linoleic acid hydroperoxide, the thiol oxidant diamide, and menadione, a generator of superoxide, amongst others. The present study analyzed the early temporal genome-wide transcriptional response of Saccharomyces cerevisiae to oxidative stress induced by the aromatic peroxide cumene hydroperoxide. The accurate dataset obtained, supported by the use of temporal controls, biological replicates and well controlled growth conditions, provided a detailed picture of the early dynamics of the process. We identified a set of genes previously not implicated in the oxidative stress response, including several transcriptional regulators showing a fast transient response, suggesting a coordinated process in the transcriptional reprogramming. We discuss the role of the glutathione, thioredoxin and reactive oxygen species-removing systems, the proteasome and the pentose phosphate pathway. A data-driven clustering of the expression patterns identified one specific cluster that mostly consisted of genes known to be regulated by the Yap1p and Skn7p transcription factors, emphasizing their mediator role in the transcriptional response to oxidants. Comparison of our results with data reported for hydrogen peroxide identified 664 genes that specifically respond to cumene hydroperoxide, suggesting distinct transcriptional responses to these two peroxides. Genes up-regulated only by cumene hydroperoxide are mainly related to the cell membrane and cell wall, and proteolysis process, while those down-regulated only by this aromatic peroxide are involved in mitochondrial function.
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