Chlamydia trachomatis infection results in a modest pro-inflammatory cytokine response and a decrease in T cell chemokine secretion in human polarized endocervical epithelial cells.
Chlamydia trachomatis infection results in a modest pro-inflammatory cytokine response and a decrease in T cell chemokine secretion in human polarized endocervical epithelial cells.
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DOI：
10.1016/j.cyto.2013.04.022
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发表时间：
2013-08
期刊：
影响因子：
3.8
通讯作者：
Quayle, Alison J.
中科院分区：
文献类型：
--
作者：
Buckner, Lyndsey R.;Lewis, Maria E.;Greene, Sheila J.;Foster, Timothy P.;Quayle, Alison J.
宫颈上皮是女性沙眼衣原体的主要宿主，生殖器感染的持续时间较长。上皮细胞通过分泌细胞因子和趋化因子来响应病原体的攻击和感染，从而起到粘膜哨兵的作用。因此，我们确定了沙眼衣原体一种常见的生殖器血清型(D)对原发样子宫内膜来源的上皮细胞的标志性细胞因子和趋化因子反应。在这些研究中，我们使用了最近建立的极化、永生化的宫颈上皮细胞模型(PolA2EN)，该模型在体外保持了体内宫颈上皮细胞的结构和功能特征，包括产生促炎细胞因子。PolA2EN细胞对沙眼衣原体感染敏感，沙眼衣原体在这些细胞中经历了一个正常的发育周期，由一步生长曲线确定。感染沙眼衣原体PolA2EN细胞的顶端和基底侧壁分泌物中IL-1α蛋白水平均升高，但这种反应在感染后72小时才出现。此外，在沙眼衣原体感染的polA2EN细胞和模拟感染的细胞中，致炎细胞因子和趋化因子IL 6、肿瘤坏死因子α和CXCL8的蛋白水平在沙眼衣原体感染后12 0h的各个时间点均无显著差异。有趣的是，沙眼衣原体感染导致T细胞趋化因子IP10和RANTES的结构性分泌显著减少，这需要沙眼衣原体的有效感染。对抗炎细胞因子的检测显示，PolA2EN细胞具有高度结构性的顶端分泌IL1ra的能力，而沙眼衣原体感染对这种分泌没有明显的调节作用。沙眼衣原体可诱导IL-11产生，但仅从基底膜诱导。这些结果表明，沙眼衣原体可以使用逃避策略来避开强大的促炎细胞因子和趋化因子反应。这些逃避策略，加上宫颈上皮细胞固有的免疫系统，可能有助于衣原体在体内建立并可能维持宫颈内微环境中的细胞内生态位。
The endocervical epithelium is a major reservoir for Chlamydia trachomatis in women, and genital infections are extended in their duration. Epithelial cells act as mucosal sentinels by secreting cytokines and chemokines in response to pathogen challenge and infection. We therefore determined the signature cytokine and chemokine response of primary-like endocervix-derived epithelial cells in response to a common genital serovar (D) of C. trachomatis. For these studies, we used a recently-established polarized, immortalized, endocervical epithelial cell model (polA2EN) that maintains, in vitro, the architectural and functional characteristics of endocervical epithelial cells in vivo including the production of pro-inflammatory cytokines. PolA2EN cells were susceptible to C. trachomatis infection, and chlamydiae in these cells underwent a normal developmental cycle as determined by a one-step growth curve. IL1α protein levels were increased in both apical and basolateral secretions of C. trachomatis infected polA2EN cells, but this response did not occur until 72 hours after infection. Furthermore, protein levels of the pro-inflammatory cytokines and chemokines IL6, TNFα and CXCL8 were not significantly different between C. trachomatis infected polA2EN cells and mock infected cells at any time during the chlamydial developmental cycle up to 120 hours post-infection. Intriguingly, C. trachomatis infection resulted in a significant decrease in the constitutive secretion of T cell chemokines IP10 and RANTES, and this required a productive C. trachomatis infection. Examination of anti-inflammatory cytokines revealed a high constitutive apical secretion of IL1ra from polA2EN cells that was not significantly modulated by C. trachomatis infection. IL-11 was induced by C. trachomatis, although only from the basolateral membrane. These results suggest that C. trachomatis can use evasion strategies to circumvent a robust pro-inflammatory cytokine and chemokine response. These evasion strategies, together with the inherent immune repertoire of endocervical epithelial cells, may aid chlamydiae in establishing, and possibly sustaining, an intracellular niche in microenvironments of the endocervix in vivo.
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影响因子：
5.8
作者：
Giles, David K.;Whittimore, Judy D.;Wyrick, Priscilla B.
通讯作者：
Wyrick, Priscilla B.
影响因子：
6.8
作者：
Dorner, AJ;Goldman, SJ;Keith, JC
通讯作者：
Keith, JC
影响因子：
3.1
作者：
ECKMANN, L;KAGNOFF, MF;FIERER, J
通讯作者：
FIERER, J
影响因子：
4.8
作者：
Fehlner-Gardiner, C;Roshick, C;McClarty, G
通讯作者：
McClarty, G
DOI：
10.1111/j.1749-6632.1994.tb44274.x
发表时间：
1994-01-01
期刊：
MICROBIAL PATHOGENESIS AND IMMUNE RESPONSE
影响因子：
--
作者：
BEATTY, WL;BELANGER, TA;BYRNE, GI
通讯作者：
BYRNE, GI