Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages.
Palmitate and minimally-modified low-density lipoprotein cooperatively promote inflammatory responses in macrophages.
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DOI：
10.1371/journal.pone.0193649
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lee SH
中科院分区：
文献类型：
--
作者：
Ann SJ;Kim KK;Cheon EJ;Noh HM;Hwang I;Yu JW;Park S;Kang SM;Manabe I;Miller YI;Kim S;Lee SH
西式饮食和环境损害的增加导致血浆饱和脂肪酸和脂蛋白氧化水平的广泛增加。本研究的目的是检查棕榈酸和最低修饰的低密度脂蛋白（mmLDL）是否对巨噬细胞活化产生累加效应。我们发现，在脂多糖（LPS）存在的情况下，用棕榈酸和mmLDL共同处理J774巨噬细胞，CXCL 2和TNF-α的分泌以及ERK和p38磷酸化均增加。此外，使用KEGG数据库对差异表达基因的分析显示，包括精氨酸-细胞因子受体相互作用在内的几种途径和基因发生了显著改变。这些结果用实时PCR验证，显示IL-6、Csf 3、IL-1β和Clec 4d的上调。目前的研究表明，棕榈酸酯和mmLDL加性增强LPS诱导的巨噬细胞活化。这些结果表明，饱和脂肪酸和氧化脂蛋白激活免疫细胞的协同机制的存在。
Increased consumption of Western-type diets and environmental insults lead to wide-spread increases in the plasma levels of saturated fatty acids and lipoprotein oxidation. The aim of this study is to examine whether palmitate and minimally modified low-density lipoprotein (mmLDL) exert an additive effect on macrophage activation. We found that CXCL2 and TNF-α secretion as well as ERK and p38 phosphorylation were additively increased by co-treatment of J774 macrophages with palmitate and mmLDL in the presence of lipopolysaccharide (LPS). Furthermore, the analysis of differentially expressed genes using the KEGG database revealed that several pathways, including cytokine-cytokine receptor interaction, and genes were significantly altered. These results were validated with real-time PCR, showing upregulation of Il-6, Csf3, Il-1β, and Clec4d. The present study demonstrated that palmitate and mmLDL additively potentiate the LPS-induced activation of macrophages. These results suggest the existence of synergistic mechanisms by which saturated fatty acids and oxidized lipoproteins activate immune cells.
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氧化脂质和脂蛋白在炎症中的上下文依赖性作用。
DOI：
10.1016/j.tem.2016.11.002
发表时间：
2017-02
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
Miller YI;Shyy JY
通讯作者：
Shyy JY
影响因子：
3.7
作者：
Choi SH;Wiesner P;Almazan F;Kim J;Miller YI
通讯作者：
Miller YI
影响因子：
7.7
作者：
Ichioka M;Suganami T;Tsuda N;Shirakawa I;Hirata Y;Satoh-Asahara N;Shimoda Y;Tanaka M;Kim-Saijo M;Miyamoto Y;Kamei Y;Sata M;Ogawa Y
通讯作者：
Ogawa Y
影响因子：
20.1
作者：
Choi SH;Harkewicz R;Lee JH;Boullier A;Almazan F;Li AC;Witztum JL;Bae YS;Miller YI
通讯作者：
Miller YI
影响因子：
64.8
作者：
Gross, Olaf;Gewies, Andreas;Ruland, Juergen
通讯作者：
Ruland, Juergen