Revealing the Specific Regulations of Brassinolide on Tomato Fruit Chilling Injury by Integrated Multi-Omics.
Revealing the Specific Regulations of Brassinolide on Tomato Fruit Chilling Injury by Integrated Multi-Omics.
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DOI：
10.3389/fnut.2021.769715
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发表时间：
2021
影响因子：
5
通讯作者：
Zuo J
中科院分区：
文献类型：
--
作者：
Bai C;Zheng Y;Watkins CB;Fu A;Ma L;Gao H;Yuan S;Zheng S;Gao L;Wang Q;Meng D;Zuo J
番茄果实在低温贮藏时易发生冷害，限制了其贮藏潜力，如果使用不适当的温度，会造成经济损失。甜菜碱（BR）是一种植物生长调节剂，已知其降低果实对CI的易感性。本研究通过转录组、代谢组和蛋白质组分析揭示了BR处理缓解番茄果实CI的调控机制。结果表明，差异表达的代谢产物主要包括氨基酸、有机酸、碳水化合物和脂类。差异表达基因（DEG）涉及植物冷胁迫反应（HSFA 3、SHSP和TPR）、果实氧化还原过程（POD、PAL和LOX）、果实质地相关基因（CESA、β-Gal和PAE）、植物激素信号转导（ACS 3、ARF和ERF）、转录因子（TCP、bHLH、加塔）。此外，差异表达的蛋白质与果实质地（CESA，PE，PL和CHI），植物氧化过程（LOX，GPX，CAT和POD），植物冷胁迫反应（HSF，HSP 20，HSP 70和HSP 90 B），植物激素信号转导（BSK 1和JAR 1）和转录因子（WRKY和MYB）。研究表明，BR通过调节α-亚麻酸代谢途径中的LOX，促进茉莉酸辅酶A（JA-CoA）的合成，抑制细胞壁和膜脂的损伤，减轻番茄果实CI症状。研究结果为进一步研究番茄果实的CI机理提供了理论依据。
Tomato fruit is susceptible to chilling injury (CI) when stored at low temperatures, limiting its storage potential, and resulting in economic loss if inappropriate temperatures are used. Brassinolide (BR) is a plant growth regulator that is known to decrease the susceptibility of fruit to CI. In this study, transcriptome, metabolome, and proteome analysis revealed the regulation mechanism of BR treatment in alleviating tomato fruit CI. The results showed that the differentially expressed metabolites mainly included amino acids, organic acids, carbohydrates, and lipids. Differentially expressed genes (DEGs) were involved in plant cold stress response (HSFA3, SHSP, and TPR), fruit redox process (POD, PAL, and LOX), related to the fruit texture (CESA, β-Gal, and PAE), plant hormone signal transduction (ACS3, ARF, and ERF,), transcription factors (TCP, bHLH, GATA). Moreover, differentially expressed proteins were associated with fruit texture (CESA, PE, PL, and CHI), plant oxidation processes (LOX, GPX, CAT, and POD), plant cold stress response (HSF, HSP20, HSP70, and HSP90B), plant hormone signal transduction (BSK1 and JAR1) and transcription factors (WRKY and MYB). Our study showed that BR alleviates CI symptoms of tomato fruit by regulating LOX in the α-linolenic acid metabolism pathway, enhancing jasmonic acid-CoA (JA-CoA) synthesis, inhibiting cell wall and membrane lipid damage. The results provided a theoretical basis for further study on the CI mechanism of tomato fruit.
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影响因子：
4.1
作者：
Kachuk, Carolyn;Stephen, Kegan;Doucette, Alan
通讯作者：
Doucette, Alan
影响因子：
8.8
作者：
Cao, Shifeng;Cai, Yuting;Zheng, Yonghua
通讯作者：
Zheng, Yonghua
影响因子：
2.6
作者：
Huan Z;Wang Y;Zhang M;Zhang X;Liu Y;Kong L;Xu J
通讯作者：
Xu J
影响因子：
4.3
作者：
Aghdam, Morteza Soleimani;Asghari, Mohammadreza;Moradbeygi, Hanieh
通讯作者：
Moradbeygi, Hanieh
影响因子：
5.6
作者：
Daher FB;Braybrook SA
通讯作者：
Braybrook SA