Commercially available angiotensin II At₂ receptor antibodies are nonspecific.
Commercially available angiotensin II At₂ receptor antibodies are nonspecific.
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DOI：
10.1371/journal.pone.0069234
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Saavedra JM
中科院分区：
文献类型：
--
作者：
Hafko R;Villapol S;Nostramo R;Symes A;Sabban EL;Inagami T;Saavedra JM
Commercially available angiotensin II AT2 receptor antibodies are widely employed for receptor localization and quantification, but they have not been adequately validated. In this study, we characterized three commercially available AT2 receptor antibodies: 2818-1 from Epitomics, sc-9040 from Santa Cruz Biotechnology, Inc., and AAR-012 from Alomone Labs. Using western blot analysis the immunostaining patterns observed were different for every antibody tested, and in most cases consisted of multiple immunoreactive bands. Identical immunoreactive patterns were present in wild-type and AT2 receptor knockout mice not expressing the target protein. In the mouse brain, immunocytochemical studies revealed very different cellular immunoreactivity for each antibody tested. While the 2818-1 antibody reacted only with endothelial cells in small parenchymal arteries, the sc-9040 antibody reacted only with ependymal cells lining the cerebral ventricles, and the AAR-012 antibody reacted only with multiple neuronal cell bodies in the cerebral cortex. Moreover, the immunoreactivities were identical in brain tissue from wild-type or AT2 receptor knockout mice. Furthermore, in both mice and rat tissue extracts, there was no correlation between the observed immunoreactivity and the presence or absence of AT2 receptor binding or gene expression. We conclude that none of these commercially available AT2 receptor antibodies tested met the criteria for specificity. In the absence of full antibody characterization, competitive radioligand binding and determination of mRNA expression remain the only reliable approaches to study AT2 receptor expression.
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影响因子：
3.6
作者：
Jositsch, Gitte;Papadakis, Tamara;Haberberger, Rainer V.;Wolff, Miriam;Wess, Juergen;Kummer, Wolfgang
通讯作者：
Kummer, Wolfgang
DOI：
10.1161/hypertensionaha.112.203679
发表时间：
2013-01
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Herrera M;Sparks MA;Alfonso-Pecchio AR;Harrison-Bernard LM;Coffman TM
通讯作者：
Coffman TM
影响因子：
64.8
作者：
ICHIKI, T;LABOSKY, PA;INAGAMI, T
通讯作者：
INAGAMI, T
影响因子：
3.6
作者：
Hamdani, Nazha;van der Velden, Jolanda
通讯作者：
van der Velden, Jolanda
影响因子：
3.6
作者：
Bodei, Serena;Arrighi, Nicola;Sigala, Sandra
通讯作者：
Sigala, Sandra