Extension of Collagen Deposition in COVID-19 Post Mortem Lung Samples and Computed Tomography Analysis Findings.
Extension of Collagen Deposition in COVID-19 Post Mortem Lung Samples and Computed Tomography Analysis Findings.
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胶原蛋白沉积在COVID-19中的扩展和计算机断层扫描分析发现。
DOI：
10.3390/ijms22147498
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发表时间：
2021-07-13
影响因子：
5.6
通讯作者：
Grillo F
中科院分区：
文献类型：
--
作者：
Ball L;Barisione E;Mastracci L;Campora M;Costa D;Robba C;Battaglini D;Micali M;Costantino F;Cittadini G;Patroniti N;Pelosi P;Fiocca R;Grillo F
肺纤维化具有特殊的计算机断层扫描(CT)表现，是晚期新冠肺炎肺炎的常见表现，其可逆性研究甚少。本研究的目的是量化新冠肺炎危重患者尸检标本中胶原沉积和充气的范围，并描述其与肺部CT定性和定量分析的相关性。取死后经支气管镜活检标本，福尔马林固定，石蜡包埋，天狼星红染色，定量胶原沉积，定义为胶原沉积大于10%的纤维化标本。肺CT图像用放射学评分进行定性分析，并在肺叶水平用计算机进行定量分析。本研究收集了10例在有创机械通气中死亡的新冠肺炎肺炎患者的30份标本。中位数[四分位数范围]的胶原伸展百分比为6.8%(4.6-16.2%)。与非纤维化患者相比，纤维化患者的定性评分较高(260vs.190(120-270)，p=0.036)，而气体分数则较低(0.46vs.0.39-0.68，p=0.047)。放射学评分>230对诊断肝纤维化有100%的敏感性(95%可信区间，CI：66.4%~100%)和66.7%的特异性(95%CI：41.0%~92.3%)，而GAS评分低于0.57分则有100%的敏感性(95%CI：66.4%~100%)和57.1%的特异性(95%CI：26.3%~88.0%)。在新冠肺炎肺炎中，肺CT图像定性和定量分析对组织病理学纤维化的诊断具有较高的敏感性，但特异性中等至低。假性纤维化的CT表现并不总是与胶原沉积增加相对应。
Lung fibrosis has specific computed tomography (CT) findings and represents a common finding in advanced COVID-19 pneumonia whose reversibility has been poorly investigated. The aim of this study was to quantify the extension of collagen deposition and aeration in postmortem cryobiopsies of critically ill COVID-19 patients and to describe the correlations with qualitative and quantitative analyses of lung CT. Postmortem transbronchial cryobiopsy samples were obtained, formalin fixed, paraffin embedded and stained with Sirius red to quantify collagen deposition, defining fibrotic samples as those with collagen deposition above 10%. Lung CT images were analyzed qualitatively with a radiographic score and quantitatively with computer-based analysis at the lobe level. Thirty samples from 10 patients with COVID-19 pneumonia deceased during invasive mechanical ventilation were included in this study. The median [interquartile range] percent collagen extension was 6.8% (4.6–16.2%). In fibrotic compared to nonfibrotic samples, the qualitative score was higher (260 (250–290) vs. 190 (120–270), p = 0.036) while the gas fraction was lower (0.46 (0.32–0.47) vs. 0.59 (0.37–0.68), p = 0.047). A radiographic score above 230 had 100% sensitivity (95% confidence interval, CI: 66.4% to 100%) and 66.7% specificity (95% CI: 41.0% to 92.3%) to detect fibrotic samples, while a gas fraction below 0.57 had 100% sensitivity (95% CI: 66.4% to 100%) and 57.1% specificity (95% CI: 26.3% to 88.0%). In COVID-19 pneumonia, qualitative and quantitative analyses of lung CT images have high sensitivity but moderate to low specificity to detect histopathological fibrosis. Pseudofibrotic CT findings do not always correspond to increased collagen deposition.
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DOI：
10.1183/09031936.00196412
发表时间：
2014-01
期刊：
The European respiratory journal
影响因子：
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作者：
Burnham EL;Janssen WJ;Riches DW;Moss M;Downey GP
通讯作者：
Downey GP
DOI：
10.1164/rccm.2106157
发表时间：
2002-06-01
影响因子：
24.7
作者：
Ichikado, K;Suga, M;Ando, M
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Ando, M
DOI：
10.1016/s2213-2600(20)30370-2
发表时间：
2020-12
期刊：
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影响因子：
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Grasselli G;Tonetti T;Protti A;Langer T;Girardis M;Bellani G;Laffey J;Carrafiello G;Carsana L;Rizzuto C;Zanella A;Scaravilli V;Pizzilli G;Grieco DL;Di Meglio L;de Pascale G;Lanza E;Monteduro F;Zompatori M;Filippini C;Locatelli F;Cecconi M;Fumagalli R;Nava S;Vincent JL;Antonelli M;Slutsky AS;Pesenti A;Ranieri VM;collaborators
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collaborators
影响因子：
9.6
作者：
González J;Benítez ID;Carmona P;Santisteve S;Monge A;Moncusí-Moix A;Gort-Paniello C;Pinilla L;Carratalá A;Zuil M;Ferrer R;Ceccato A;Fernández L;Motos A;Riera J;Menéndez R;Garcia-Gasulla D;Peñuelas O;Bermejo-Martin JF;Labarca G;Caballero J;Torres G;de Gonzalo-Calvo D;Torres A;Barbé F;CIBERESUCICOVID Project (COV20/00110, ISCIII)
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CIBERESUCICOVID Project (COV20/00110, ISCIII)
DOI：
10.1007/s00428-020-02934-1
发表时间：
2021-03
期刊：
Virchows Archiv : an international journal of pathology
影响因子：
--
作者：
Barisione E;Grillo F;Ball L;Bianchi R;Grosso M;Morbini P;Pelosi P;Patroniti NA;De Lucia A;Orengo G;Gratarola A;Verda M;Cittadini G;Mastracci L;Fiocca R
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Fiocca R