Antibody responses of healthy adults to the p27 peptide of respiratory syncytial virus fusion protein.
Antibody responses of healthy adults to the p27 peptide of respiratory syncytial virus fusion protein.
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DOI：
10.1016/j.vaccine.2021.11.087
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发表时间：
2022-01-24
期刊：
影响因子：
5.5
通讯作者：
Piedra PA
中科院分区：
文献类型：
--
作者：
Blunck BN;Aideyan L;Ye X;Avadhanula V;Ferlic-Stark L;Zechiedrich L;Gilbert BE;Piedra PA
呼吸道合胞病毒（RSV）融合(F)蛋白经历两次furin裂解事件成为融合能力，导致27个氨基酸的肽的释放（p27）。最近的研究表明，F蛋白的p27区域是幼儿免疫优势抗原。在这项研究中，我们评估了成人自然RSV再感染后血清抗体对p27肽的反应动力学。在2018-2019年RSV季节，德克萨斯州休斯顿招募了19名65岁以下的健康成年人。在三次研究访问中收集血液，通过中和抗体的变化定义RSV感染状态，结果分为三组：未感染（n=12），急性感染（n=4）和最近感染（n=3）。采用酶联免疫吸附法检测血清抗RSV/A和RSV/B p27肽的IgG和IgA抗体，采用p27竞争抗体法检测血清p27样抗体。每次研究访问时，所有受试者均检测到抗p27抗体。测量的IgG和IgA抗p27抗体水平与之前在该队列中描述的其他RSV位点特异性和中和抗体反应具有相同的模式：未感染组在研究期间反应稳定，急性感染组在RSV感染后显著增加，而最近感染组在研究期间抗p27抗体下降。这些结果表明，在自然RSV再感染后，F蛋白的p27区域产生抗体，并表明一些F蛋白可能在病毒粒子表面处于部分裂解状态，扩展了之前的假设，即所有p27都是翻译后释放的，而不存在于成熟的F上。此外，每次研究访问时，抗体对RSV/B的反应明显低于对RSV/ a p27的反应（1.4 - 1.5倍）。尽管这是一次RSV/B为主的暴发。了解RSV/A和RSV/B p27抗体反应大小差异的机制可能有助于我们对F蛋白细胞内加工的理解。
The respiratory syncytial virus (RSV) fusion (F) protein undergoes two furin-cleavage events to become fusion competent, resulting in the release of a twenty-seven amino acid peptide (p27). Recent studies indicate that the p27 region of the F protein was an immunodominant antigen in young children. In this study, we evaluated the kinetics of the serum antibody response to the p27 peptide following natural RSV reinfection in adults. Nineteen healthy adults under sixty-five years of age were enrolled during the 2018–2019 RSV season in Houston, TX. Blood was collected at three study visits and RSV infection status was defined by changes in neutralizing antibody resulting in three groups: uninfected (n=12), acutely infected (n=4), and recently infected (n=3). Serum IgG and IgA antibodies against RSV/A and RSV/B p27 peptides were measured by enzyme-linked immunosorbent assays, and serum p27-like antibodies were detected by a p27 competitive antibody assay. Anti-p27 antibodies were detected in all subjects at each study visit. The measured IgG and IgA anti-p27 antibody levels followed the same pattern as other RSV site-specific and neutralizing antibody responses described for this cohort previously: the uninfected group had stable responses for the duration of the study period, the acutely infected group had a significant increase following RSV infection, and the recently infected group had a decrease in anti-p27 antibody during the study period. These results indicate that antibodies to the p27 region of the F protein are generated following natural RSV reinfection and suggest that some of the F protein is potentially in a partially cleaved state on the surface of virions, expanding on the previous assumption that all of p27 is post-translationally released and not present on mature F. Additionally, antibody responses were significantly lower (1.4–1.5-fold) toward RSV/B than to RSV/A p27 at each study visit, despite being an RSV/B dominant outbreak. Understanding the mechanism for the differences in the magnitude of the RSV/A and RSV/B p27 antibody response may enhance our understanding of the intracellular processing of the F protein.
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发表时间：
2017-09-02
期刊：
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影响因子：
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作者：
Shi T;McAllister DA;O'Brien KL;Simoes EAF;Madhi SA;Gessner BD;Polack FP;Balsells E;Acacio S;Aguayo C;Alassani I;Ali A;Antonio M;Awasthi S;Awori JO;Azziz-Baumgartner E;Baggett HC;Baillie VL;Balmaseda A;Barahona A;Basnet S;Bassat Q;Basualdo W;Bigogo G;Bont L;Breiman RF;Brooks WA;Broor S;Bruce N;Bruden D;Buchy P;Campbell S;Carosone-Link P;Chadha M;Chipeta J;Chou M;Clara W;Cohen C;de Cuellar E;Dang DA;Dash-Yandag B;Deloria-Knoll M;Dherani M;Eap T;Ebruke BE;Echavarria M;de Freitas Lázaro Emediato CC;Fasce RA;Feikin DR;Feng L;Gentile A;Gordon A;Goswami D;Goyet S;Groome M;Halasa N;Hirve S;Homaira N;Howie SRC;Jara J;Jroundi I;Kartasasmita CB;Khuri-Bulos N;Kotloff KL;Krishnan A;Libster R;Lopez O;Lucero MG;Lucion F;Lupisan SP;Marcone DN;McCracken JP;Mejia M;Moisi JC;Montgomery JM;Moore DP;Moraleda C;Moyes J;Munywoki P;Mutyara K;Nicol MP;Nokes DJ;Nymadawa P;da Costa Oliveira MT;Oshitani H;Pandey N;Paranhos-Baccalà G;Phillips LN;Picot VS;Rahman M;Rakoto-Andrianarivelo M;Rasmussen ZA;Rath BA;Robinson A;Romero C;Russomando G;Salimi V;Sawatwong P;Scheltema N;Schweiger B;Scott JAG;Seidenberg P;Shen K;Singleton R;Sotomayor V;Strand TA;Sutanto A;Sylla M;Tapia MD;Thamthitiwat S;Thomas ED;Tokarz R;Turner C;Venter M;Waicharoen S;Wang J;Watthanaworawit W;Yoshida LM;Yu H;Zar HJ;Campbell H;Nair H;RSV Global Epidemiology Network
通讯作者：
RSV Global Epidemiology Network