Estrogen-dependent uterine secretion of osteopontin activates blastocyst adhesion competence.
Estrogen-dependent uterine secretion of osteopontin activates blastocyst adhesion competence.
复制标题
DOI：
10.1371/journal.pone.0048933
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Imakawa K
中科院分区：
文献类型：
--
作者：
Chaen T;Konno T;Egashira M;Bai R;Nomura N;Nomura S;Hirota Y;Sakurai T;Imakawa K
胚胎植入是一个高度协调的过程，涉及胚泡-子宫相互作用。这一过程被限制在妊娠期间的一个确定的时间间隔内，称为“胚胎植入接受性窗口”。在小鼠中，这一接受期由卵巢雌激素控制，并涉及胚泡粘附能力和子宫接受性的协调。协调胚泡粘附能力和子宫容受性的机制在很大程度上是未知的。在这里，我们表明，卵巢雌激素间接调节囊胚粘附能力。胚泡粘附能力的获得归因于整合素活化（例如粘附复合物的形成）而不是从头整合素合成。骨桥蛋白（OPN）是一种雌激素依赖的子宫内膜腺分泌因子，参与激活囊胚黏附能力。通过黏着斑激酶（FAK）和磷脂酰肌醇3-激酶（PI 3 K）依赖性信号通路介导OPN处理的囊胚中粘附复合物组装增加。这些发现首次明确了雌激素依赖性途径协调胚泡粘附能力和子宫容受性的特定调控成分。
Embryo implantation is a highly orchestrated process that involves blastocyst-uterine interactions. This process is confined to a defined interval during gestation referred to as the “window of embryo implantation receptivity”. In mice this receptive period is controlled by ovarian estrogen and involves a coordination of blastocyst adhesion competence and uterine receptivity. Mechanisms coordinating the acquisition of blastocyst adhesion competence and uterine receptivity are largely unknown. Here, we show that ovarian estrogen indirectly regulates blastocyst adhesion competence. Acquisition of blastocyst adhesion competence was attributed to integrin activation (e.g. formation of adhesion complexes) rather than de novo integrin synthesis. Osteopontin (OPN) was identified as an estrogen-dependent uterine endometrial gland secretory factor responsible for activating blastocyst adhesion competence. Increased adhesion complex assembly in OPN-treated blastocysts was mediated through focal adhesion kinase (FAK)- and phosphatidylinositol 3-kinase (PI3K)-dependent signaling pathways. These findings define for the first time specific regulatory components of an estrogen-dependent pathway coordinating blastocyst adhesion competence and uterine receptivity.
登录
查看更多内容
影响因子：
4
作者：
Humphries JD;Byron A;Humphries MJ
通讯作者：
Humphries MJ
DOI：
10.1083/jcb.106.2.441
发表时间：
1988-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nomura S;Wills AJ;Edwards DR;Heath JK;Hogan BL
通讯作者：
Hogan BL
DOI：
10.1073/pnas.83.18.6751
发表时间：
1986-09-01
影响因子：
11.1
作者：
ARMANT, DR;KAPLAN, HA;LENNARZ, WJ
通讯作者：
LENNARZ, WJ
DOI：
10.1083/jcb.200703036
发表时间：
2007-12-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Humphries JD;Wang P;Streuli C;Geiger B;Humphries MJ;Ballestrem C
通讯作者：
Ballestrem C
影响因子：
3.5
作者：
Fujita, Y;Kitagawa, M;Harigaya, K
通讯作者：
Harigaya, K