An observational, laboratory-based study of outbreaks of middle East respiratory syndrome coronavirus in Jeddah and Riyadh, kingdom of Saudi Arabia, 2014.
An observational, laboratory-based study of outbreaks of middle East respiratory syndrome coronavirus in Jeddah and Riyadh, kingdom of Saudi Arabia, 2014.
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DOI：
10.1093/cid/ciu812
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发表时间：
2015-02-01
期刊：
影响因子：
--
通讯作者：
Memish ZA
中科院分区：
文献类型：
--
作者：
Drosten C;Muth D;Corman VM;Hussain R;Al Masri M;HajOmar W;Landt O;Assiri A;Eckerle I;Al Shangiti A;Al-Tawfiq JA;Albarrak A;Zumla A;Rambaut A;Memish ZA
2014年春天，吉达爆发了中东呼吸综合征冠状病毒，引起了人们对病毒传播性变化的担忧。病毒的功能检查和诊断实验室数据分析表明，病原体是由生物学上未改变的病毒在医院内传播引起的。 背景：2014年春季，沙特阿拉伯各地报告的中东呼吸综合征冠状病毒感染人数突然增加，重点是吉达。 解释暴发模式的假设包括加强监测、增加人畜共患病传播、医院内传播和病毒传播能力的变化，以及诊断实验室人为因素。 方法：分析吉达地区实验室的诊断结果。 对吉达爆发的病毒和同一时间在利雅得、阿尔-哈杰和麦地那发生的病毒进行了全部或部分测序。从其他病毒中确定了吉达爆发特有的一组4个单核苷酸多态性。从利雅得和吉达分离病毒，并在细胞培养中进行研究。 结果：每天收到多达481份样本进行逆转录聚合酶链反应（RT-PCR）检测。 实验室能力评估表明阳性和阴性结果是可靠的。在吉达爆发期间，168个阳性检测样本中有49%来自法赫德国王医院。所有来自吉达的病毒都是单系的和相似的，而来自利雅得的病毒是并系的和多样的。在利雅得发现了一个与医院有关的传播集群，印第安纳州（美国）和荷兰的病例属于该集群。在利雅得发现了一种吉达型病毒，与吉达的旅行史相匹配。代表吉达和利雅得暴发的病毒分离株在复制、干扰素应答逃逸或血清中和方面与MERS CoV EMC/2012没有差异。 结论：在吉达暴发期间，病毒脱落和病毒功能没有显著变化。 这些结果表明，暴发是由与医院传播有关的生物学上不变的病毒引起的。
In spring 2014, an outbreak of Middle East respiratory syndrome coronavirus in Jeddah caused conjectures about changes in viral transmissibility. Functional examination of viruses and analyses of diagnostic laboratory data suggest causation by nosocomial transmission of a biologically unchanged virus. Background. In spring 2014, a sudden rise in the number of notified Middle East respiratory syndrome coronavirus (MERS-CoV) infections occurred across Saudi Arabia with a focus in Jeddah. Hypotheses to explain the outbreak pattern include increased surveillance, increased zoonotic transmission, nosocomial transmission, and changes in viral transmissibility, as well as diagnostic laboratory artifacts. Methods. Diagnostic results from Jeddah Regional Laboratory were analyzed. Viruses from the Jeddah outbreak and viruses occurring during the same time in Riyadh, Al-Kharj, and Madinah were fully or partially sequenced. A set of 4 single-nucleotide polymorphisms distinctive to the Jeddah outbreak were determined from additional viruses. Viruses from Riyadh and Jeddah were isolated and studied in cell culture. Results. Up to 481 samples were received per day for reverse transcription polymerase chain reaction (RT-PCR) testing. A laboratory proficiency assessment suggested positive and negative results to be reliable. Forty-nine percent of 168 positive-testing samples during the Jeddah outbreak stemmed from King Fahd Hospital. All viruses from Jeddah were monophyletic and similar, whereas viruses from Riyadh were paraphyletic and diverse. A hospital-associated transmission cluster, to which cases in Indiana (United States) and the Netherlands belonged, was discovered in Riyadh. One Jeddah-type virus was found in Riyadh, with matching travel history to Jeddah. Virus isolates representing outbreaks in Jeddah and Riyadh were not different from MERS-CoV EMC/2012 in replication, escape of interferon response, or serum neutralization. Conclusions. Virus shedding and virus functions did not change significantly during the outbreak in Jeddah. These results suggest the outbreaks to have been caused by biologically unchanged viruses in connection with nosocomial transmission.
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影响因子：
4.8
作者：
Herzog, Petra;Drosten, Christian;Mueller, Marcel A.
通讯作者：
Mueller, Marcel A.
影响因子：
39.2
作者：
Leung, GM;Hedley, AJ;Lam, TH
通讯作者：
Lam, TH
由鼻咽拭子筛查的医疗工人中严重急性呼吸道综合征相关的冠状病毒的定殖。
影响因子：
9.6
作者：
Ho HT;Chang MS;Wei TY;Hsieh WS;Hung CC;Yang HM;Lu YT
通讯作者：
Lu YT
DOI：
10.1056/nejmoa1306742
发表时间：
2013-08-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
Assiri A;McGeer A;Perl TM;Price CS;Al Rabeeah AA;Cummings DA;Alabdullatif ZN;Assad M;Almulhim A;Makhdoom H;Madani H;Alhakeem R;Al-Tawfiq JA;Cotten M;Watson SJ;Kellam P;Zumla AI;Memish ZA;KSA MERS-CoV Investigation Team
通讯作者：
KSA MERS-CoV Investigation Team
DOI：
10.1016/s1473-3099(13)70154-3
发表时间：
2013-09
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Drosten C;Seilmaier M;Corman VM;Hartmann W;Scheible G;Sack S;Guggemos W;Kallies R;Muth D;Junglen S;Müller MA;Haas W;Guberina H;Röhnisch T;Schmid-Wendtner M;Aldabbagh S;Dittmer U;Gold H;Graf P;Bonin F;Rambaut A;Wendtner CM
通讯作者：
Wendtner CM