A comparative UPLC-Q-Orbitrap-MS untargeted metabolomics investigation of different parts of Clausena lansium (Lour.) Skeels.

A comparative UPLC-Q-Orbitrap-MS untargeted metabolomics investigation of different parts of Clausena lansium (Lour.) Skeels.
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DOI:
10.1002/fsn3.1841
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发表时间:
2020-11
影响因子:
3.9
通讯作者:
Zeng J
Zeng J
中科院分区:
农林科学3区
文献类型:
--
作者:
Fan R;Peng C;Zhang X;Qiu D;Mao G;Lu Y;Zeng J

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本研究采用非靶向大规模植物代谢组学方法(UPLC-Q-Orbitrap-MS)对黄皮的叶、皮、花、皮、果肉和种子进行了化学分析比较。Skeels(又称“wampee”)。共鉴定出364种代谢物,通过多变量统计分析选择了62种潜在的生物标志物。系统聚类分析表明,所选的生物标志物是黄皮不同部位间显著差异的代谢产物。代谢途径分析表明,“黄酮和黄酮醇合成”和“异喹啉生物碱生物合成”途径明显富集。本研究为黄皮不同组织中功能成分的分离鉴定和代谢生物合成途径的详细阐明提供了重要信息。对黄皮的叶、皮、花、皮、果肉和种子的化学成分进行了比较研究。本研究为黄皮不同组织中功能成分的分离鉴定和代谢生物合成途径的详细阐明提供了重要信息。
In this study, the non‐targeted large‐scale plant metabolomics (UPLC‐Q‐Orbitrap‐MS) was performed for the comparison of chemical profiling of the leaves, barks, flowers, peels, pulps, and seeds of Clausena lansium (Lour.) Skeels (called “wampee”). A total of 364 metabolites were identified, and 62 potential biomarkers were selected by the multivariate statistical analysis. Hierarchical cluster analysis suggested that the selected biomarkers were significant differential metabolites among various parts of wampee. Metabolic pathway analysis showed a significant enrichment of the “Flavone and flavonol synthesis” and “Isoquinoline alkaloid biosynthesis” pathway. This study provides important information for the isolation and identification of functional components from different tissues of wampee and the metabolic biosynthesis pathway elucidation in detail. The comparison of chemical profiling of the leaves, barks, flowers, peels, pulps, and seeds of wampee was investigated. This study provides important information for the isolation and identification of functional components from different tissues of wampee and the metabolic biosynthesis pathway elucidation in detail.
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