Whole blood FPR1 mRNA expression predicts both non-small cell and small cell lung cancer.
Whole blood FPR1 mRNA expression predicts both non-small cell and small cell lung cancer.
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DOI：
10.1002/ijc.31245
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发表时间：
2018-06-01
影响因子：
6.4
通讯作者：
Mallery DW
中科院分区：
文献类型：
--
作者：
Morris S;Vachani A;Pass HI;Rom WN;Ryden K;Weiss GJ;Hogarth DK;Runger G;Richards D;Shelton T;Mallery DW
虽然早期肺癌的长期生存率很高，但大多数病例在晚期被诊断出来，这可能会对生存率产生负面影响。我们的目标是为早期肺癌设计一种简单的单一生物标志物血液检测，该检测对临床前变量具有鲁棒性，并且可以在临床上轻松实施。从29名患者的训练组和260名患者的验证组中收集PAXgene管中的全血，其中58名患者的样本是在专门用于我们研究的临床试验中前瞻性收集的。提取RNA后，通过自动化一步Taqman RT-PCR测定定量FPR 1和参考基因的表达。全血中FPR 1 mRNA水平升高预测肺癌状态的灵敏度为55%，所有验证标本的特异性为87%。前瞻性采集的标本具有显著更高的68%的灵敏度和89%的特异性。良性结节患者的结果与健康志愿者相似。我们的检测结果与除肺癌诊断外的任何临床特征之间均无有意义的相关性。全血中FPR 1 mRNA水平可以预测肺癌的存在。使用此作为阳性肺癌筛查的反射测试，计算机断层扫描有可能增加阳性预测值。该标记物可以利用现成的设备和试剂在自动化过程中轻松测量。进一步的工作是合理的，以解释这种生物标志物的来源。 有什么新消息吗？ 有几项肺癌筛查试验评估了成像对改善肺癌患者生存结局的潜在益处。虽然低剂量计算机断层扫描（CT）筛查可降低死亡率，但其假阳性率为96.4%。一个潜在的策略，以改善筛选可能是识别额外的工具，提高识别假阳性。使用前瞻性收集的全血样本，作者表明FPR 1 mRNA表达升高具有68%的灵敏度和89%的特异性。这种单一的生物标志物血液检测，可以很容易地在临床上实施，可能会增加检测肺癌的阳性预测值。
While long‐term survival rates for early‐stage lung cancer are high, most cases are diagnosed in later stages that can negatively impact survival rates. We aim to design a simple, single biomarker blood test for early‐stage lung cancer that is robust to preclinical variables and can be readily implemented in the clinic. Whole blood was collected in PAXgene tubes from a training set of 29 patients, and a validation set of 260 patients, of which samples from 58 patients were prospectively collected in a clinical trial specifically for our study. After RNA was extracted, the expressions of FPR1 and a reference gene were quantified by an automated one‐step Taqman RT‐PCR assay. Elevated levels of FPR1 mRNA in whole blood predicted lung cancer status with a sensitivity of 55% and a specificity of 87% on all validation specimens. The prospectively collected specimens had a significantly higher 68% sensitivity and 89% specificity. Results from patients with benign nodules were similar to healthy volunteers. No meaningful correlation was present between our test results and any clinical characteristic other than lung cancer diagnosis. FPR1 mRNA levels in whole blood can predict the presence of lung cancer. Using this as a reflex test for positive lung cancer screening computed tomography scans has the potential to increase the positive predictive value. This marker can be easily measured in an automated process utilizing off‐the‐shelf equipment and reagents. Further work is justified to explain the source of this biomarker. What's new? There have been several lung cancer screening trials evaluating the potential benefit of imaging for improving survival outcomes in lung cancer patients. While low‐dose computed tomography (CT) screening reduces mortality, it yields a 96.4% false‐positive rate. A potential strategy to improve screening may be the identification of additional tools that improve identification of false positives. Using prospectively collected whole blood samples, here the authors show that elevated FPR1 mRNA expression has a 68% sensitivity and 89% specificity. This single biomarker blood test, which can be readily implemented in the clinic, may increase the positive predictive value of detecting lung cancer.
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影响因子：
4.7
作者：
Hanssen A;Loges S;Pantel K;Wikman H
通讯作者：
Wikman H
影响因子：
5.1
作者：
Lin CC;Huang WL;Wei F;Su WC;Wong DT
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作者：
Weiss GJ;Waypa J;Blaydorn L;Coats J;McGahey K;Sangal A;Niu J;Lynch CA;Farley JH;Khemka V
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影响因子：
45.3
作者：
Sin, Don D.;Tammemagi, C. Martin;Taguchi, Ayumu
通讯作者：
Taguchi, Ayumu
影响因子：
64.8
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通讯作者：
Thomas RK