Identification and profiles of microRNAs in different development stages of miniature pig secondary palate.
Identification and profiles of microRNAs in different development stages of miniature pig secondary palate.
复制标题
小型猪次级上腭不同发育阶段的 microRNA 鉴定及谱分析
DOI：
10.1016/j.ygeno.2021.06.009
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发表时间：
2021-06
期刊：
影响因子：
4.4
通讯作者：
Juan Du
中科院分区：
文献类型：
--
作者：
Ying Xu;Dong Yuan;Zhipeng Fan;Songlin Wang;Juan Du
腭裂是最常见的颅面畸形出生缺陷之一。小型猪(Sus Scrofa)是探索人类味觉发育的有价值的替代大型动物模型。目前，小型猪从胚胎30天到50天的腭裂发生过程中的microRNA(MiRNA)的表达谱已被鉴定。共鉴定出2044个已知miRNAs和192个新miRNAs。利用基因本体论功能和京都基因百科全书及基因组通径分析鉴定其潜在靶基因的功能特征。MiRNAs在不同发育阶段的表达水平不同。用Short-Time-Serial Expression Miner软件研究E30-50的miRNAs的表达模式，将所有miRNAs聚为20个图谱。从E30-50开始，miRNAs表达降低(剖面0)/增强(剖面19)的剖面是腭裂发生的主要模式。通过加权相关网络分析，确定了SSC-miR-98、SSC-miR-27A_R和SSC-miR-150等4个重要模块的HUB基因，它们可能是调节腭部发育的新的潜在靶点。这些数据有望在进一步的研究中提高对腭部发育和腭裂病因的理解。
Cleft palate is one of the most frequent craniofacial malformation birth defects. Miniature pigs (Sus scrofa) are a valuable alternative large animal model to explore human palate development. Presently, the microRNA (miRNA) expression profiles in miniature pigs during palatogenesis from embryonic day (E) 30 to 50 were identified. A total of 2044 known miRNAs and 192 novel miRNAs were identified. The functional characteristics of their potential target genes were identified using Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway analysis. MiRNAs displayed diverse expression levels among the different stages. Using Short Time-series Expression Miner software to investigate the expression patterns of miRNAs from E30–50, all miRNAs were clustered into 20 profiles. The profiles showing miRNAs expression decreased (profile 0)/increased (profile 19) from E30–50 were the main patterns during palatogenesis. Hub genes of four significant modules were identified by weighted correlation network analysis, including ssc-miR-98, ssc-miR-27a_R + 1, and ssc-miR-150, etc. which might be novel potential targets for regulating palate development. The data are expected to improve the understanding of palate development and the etiology of cleft palate in further studies.
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通讯作者：
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